Membrane filters are core materials for water treatment, reuse, and desalination. However, their fouling caused by biofilm growth is a major challenge, needing resolution. Here, we report the new strategies and efficacy of anti-biofouling membranes fabricated through the immobilization of quorum quenching (QQ) bacterial strains (Rhodococcus sp. BH4). The QQ cells are not attached to the membrane by direct phase inversion. However, they anchor to the membrane with its incubation in the presence of hydrophilic polymers (polyvinyl alcohol and alginate) as evidenced in the spectroscopic and morphological observations. Membrane filters with live QQ bacteria can degrade a signal molecule (N-octanoyl-l-homoserine lactone) with the first-order rate constant of 0.58–0.82 h⁻¹ and inhibit the growth of a biofilm-forming bacterium (PAO1) and its secretion of biopolymers. The QQ membrane demonstrates its significant anti-biofouling effect in bioreactors for treating synthetic wastewater (i.e., membrane fouling was delayed by 57–67% compared to the naked membrane), although the initial water permeabilities are reduced with surface modification by 34–47% compared to pristine condition. The findings of this work bring the broad potential for material fabrication requiring biofouling control, encountered in our daily lives and mechanical, marine, and medical industries in addition to membrane filters.

膜过滤器是水处理、回用和海水淡化的核心材料。然而，由生物膜生长引起的污染是一个重大挑战，需要解决。在这里，我们报告了通过固定群体淬灭 (QQ) 细菌菌株 ( Rhodococcus sp. BH4)制造的抗生物污垢膜的新策略和功效。QQ 细胞不通过直接转相连接到膜上。然而，正如光谱和形态观察所证明的那样，它们在亲水聚合物（聚乙烯醇和藻酸盐）存在的情况下通过孵育固定在膜上。带有活 QQ 细菌的膜过滤器可以降解信号分子（N-辛酰基-l-高丝氨酸内酯），一级速率常数为 0.58–0.82 h ^-1并抑制生物膜形成细菌 (PAO1) 的生长及其生物聚合物的分泌。QQ 膜在用于处理合成废水的生物反应器中表现出显着的抗生物污染效果（即，与裸膜相比，膜污染延迟了 57-67%），尽管初始水渗透率随着表面改性降低了 34-47%与原始状态相比。这项工作的发现为需要生物污染控制的材料制造带来了广泛的潜力，除了膜过滤器之外，我们的日常生活和机械、海洋和医疗行业也会遇到这些问题。