In this work, the effects of four supercritical carbon dioxide (ScCO₂) conditions potentially conductive to sterilization, on physicochemical characteristics of a liposome formulation containing apigenin (AG) were investigated. These conditions were: C1 (ScCO₂/70 °C/150 bar/240 min), C2 (ScCO₂/0.25% water/ 0.15% H₂O₂)/ 0.5% acetic anhydride/38 °C/85 bar/45 min), C3 (ScCO₂/0.08% peracetic acid/35 °C/104 bar/180 min) and C4 (ScCO₂/ 200 ppm H₂O₂/40 °C/270 bar/90 min). The results showed changes in color and a decrease in pH value of liposomes suspension after treatment with all tested conditions. Moreover, a decrease in liposome size with C1, C2 and C3 and an increase in the PDI with C1 and C3 were observed. For zeta potential, a decrease with C1, and an increase with C2 and C3 were found. Concerning the encapsulated AG, a moderate decrease, a strong decrease and a total disappearance were noted with conditions C1, C2 and C3 respectively.

在这项工作中，研究了四种可能促进灭菌的超临界二氧化碳 (ScCO ₂ ) 条件对含有芹菜素 (AG) 的脂质体制剂的物理化学特性的影响。这些条件为：C1 (ScCO ₂ /70 °C/150 bar/240 min)、C2 (ScCO ₂ /0.25% 水/0.15% H ₂ O ₂ ) /0.5%乙酸酐/38 °C/85 bar/45 min)、C3 (ScCO ₂ /0.08% 过氧乙酸/35 °C/104 bar/180 min) 和 C4 (ScCO ₂ / 200 ppm H ₂ O ₂/40 °C/270 巴/90 分钟）。结果显示在所有测试条件下处理后脂质体悬浮液的颜色变化和pH值降低。此外，观察到 C1、C2 和 C3 的脂质体大小减小，以及 C1 和 C3 的 PDI 增加。对于 zeta 电位，发现 C1 降低，C2 和 C3 增加。关于封装的 AG，分别在条件 C1、C2 和 C3 下观察到适度减少、强烈减少和完全消失。