Construction of a Fusellovirus with a Minimal Set of Genes,ACS Synthetic Biology

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Construction of a Fusellovirus with a Minimal Set of Genes
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2021-09-20 , DOI: 10.1021/acssynbio.1c00232
Junxia Zhang _{1,

2} , Li Huang _{1,

2}

Affiliation

A large number of fuselloviruses have been found in acidic hot springs around the globe. They share a set of highly conserved genes (core genes) and possess a varying number of less-conserved genes (non-core genes). However, the functions of most of these genes are unknown. Recent studies show that as many as half of these genes tolerate mutation. In this study, we conducted a genetic analysis on Saccharolobus spindle-shaped virus 22 (SSV22), an alphafusellovirus with fewer open reading frames (ORFs) than most of the isolated fuselloviruses. Both deletion and frame-shift mutations were introduced into nearly all of the 26 ORFs of the viral genome. A total of 17 ORFs were indispensable, and two additional ORFs were required for the optimal infectivity of the virus. Deletion of either VP2 or VP3, the two structural proteins, did not affect the morphology or infectivity of the virus. An infectious SSV22 derivative carrying a minimal genome of 20 ORFs was obtained. The SSV22 capsid was capable of accommodating a genome as large as ∼18 kb, or ∼7 kb larger than that of the wild-type virus. The viral capsid varied in both the length and width, but not in shape, with the size of the genome. Our results will facilitate the analysis of crucial protein–protein interactions between SSV22 and the host during viral infection and help explore the use of SSV22 as a vector for DNA delivery in potential applications.

中文翻译：

构建具有最少基因组的梭状病毒

在全球的酸性温泉中发现了大量的梭状病毒。它们共享一组高度保守的基因（核心基因）并拥有数量不等的保守基因（非核心基因）。然而，大多数这些基因的功能是未知的。最近的研究表明，多达一半的这些基因可以耐受突变。在这项研究中，我们对Saccharolobus进行了遗传分析纺锤形病毒 22 (SSV22)，一种 α 梭状病毒，其开放阅读框 (ORF) 少于大多数分离的梭状病毒。缺失和移码突变都被引入病毒基因组的几乎所有 26 个 ORF。总共 17 个 ORF 是必不可少的，并且需要两个额外的 ORF 才能使病毒具有最佳感染性。VP2 或 VP3 这两种结构蛋白的缺失不影响病毒的形态或传染性。获得了携带 20 个 ORF 的最小基因组的传染性 SSV22 衍生物。SSV22 衣壳能够容纳大至 18 kb 的基因组，或比野生型病毒大 7 kb。病毒衣壳的长度和宽度都不同，但形状不随基因组的大小而变化。

更新日期：2021-10-15

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