Saliva is an appropriate specimen for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) diagnosis. The possibility of pooling samples of saliva, using non-invasive bibula strips for sampling, was explored employing Bovine coronavirus (BCoV) spiked saliva. In laboratory, up to 30 saliva-soaked strips were pooled in a single tube with 2 mL of medium. After quick adsorption with the medium and vortexing, the liquid was collected and tested with a quantitative molecular assay to quantify viral RNA genome copies. On testing of single and pooled strips, the difference between the median threshold cycles (Ct) value of test performed on the single positive saliva sample and the median Ct value obtained on the pool of 30 strips, was 3.21 cycles. Saliva pooling with bibula strips could allow monitoring of COVID-19 on a large scale, reducing costs for the health bodies in terms of medical material and skilled personnel. Finally, saliva sampling is noninvasive and less traumatic than nasopharyngeal swabs and can be self-collected.

唾液是诊断严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 的合适标本。使用牛冠状病毒探索了使用非侵入性 bibula 条采集唾液样本的可能性(BCoV) 唾液。在实验室中，将多达 30 个唾液浸泡的条带汇集在一个装有 2 mL 培养基的试管中。在用培养基快速吸附和涡旋后，收集液体并用定量分子测定法进行测试，以量化病毒 RNA 基因组拷贝。在测试单个和合并的条带时，对单个阳性唾液样本进行的测试的中值阈值循环 (Ct) 值与在 30 个条带的池中获得的中值 Ct 值之间的差异为 3.21 个循环。使用 bibula 条收集唾液可以大规模监测 COVID-19，从而降低卫生机构在医疗材料和技术人员方面的成本。最后，唾液采样是无创的，比鼻咽拭子的创伤更小，可以自行收集。