SRO (SIMILAR TO RCD ONE) is a family of plant-specific small molecule proteins that play an important role in plant growth and development and environmental responses. However, SROs still lack systematic characterization in tomato. Based on bioinformatics methods, SRO family genes were identified and characterized from cultivated tomatoes and several wild tomatoes. qRT-PCR was used to study the expression of SRO gene in cultivated tomatoes. Phylogenetic and evolutionary analyses showed that SRO genes in angiosperms share a common ancestor and that the number of SRO family members changed as plants diverged and evolved. Cultivated tomato had six SRO members, five of which still shared some degree of identity with the ancestral SRO genes. Genetic structure and physicochemical properties showed that tomato SRO genes were highly conserved with chromosomal distribution. They could be divided into three groups based on exon-intron structure, and cultivated tomato contained only two of these subclades. A number of hormonal, light and abiotic stress-responsive cis-regulatory elements were identified from the promoter of the tomato SRO gene, and they also interacted with a variety of stress-responsive proteins and microRNAs. RNA-seq analysis showed that SRO genes were widely expressed in different tissues and developmental stages of tomato, with significant tissue-specific features. Expression analysis also showed that SRO genes respond significantly to high temperature and salt stress and mediate the tomato hormone regulatory network. These results provide a theoretical basis for further investigation of the functional expression of tomato SRO genes and provide potential genetic resources for tomato resistance breeding.

SRO（类似于 RCD ONE）是一个植物特异性小分子蛋白家族，在植物生长发育和环境响应中起重要作用。然而，SROs 在番茄中仍然缺乏系统的表征。基于生物信息学方法，SRO从栽培番茄和几种野生番茄中鉴定和表征了家族基因。qRT-PCR 用于研究SRO栽培番茄中的基因。系统发育和进化分析表明，SRO 被子植物中的基因共享一个共同的祖先，并且 SRO随着植物的分化和进化，家庭成员发生了变化。栽培番茄有六个SRO 成员，其中五人仍然与祖先有某种程度的认同 SRO基因。遗传结构和理化性质表明番茄SRO基因在染色体分布上高度保守。它们可以根据外显子-内含子结构分为三组，而栽培番茄仅包含其中的两个亚支。许多激素、光和非生物应激反应顺式- 从番茄的启动子中鉴定出调节元件 SRO基因，并且它们还与多种应激反应蛋白和微小 RNA 相互作用。RNA-seq 分析表明，SRO基因在番茄的不同组织和发育阶段广泛表达，具有显着的组织特异性特征。表达分析还表明SRO基因对高温和盐胁迫显着响应并介导番茄激素调节网络。这些结果为进一步研究番茄的功能表达提供了理论依据。SRO 基因，为番茄抗性育种提供潜在的遗传资源。