Zinc-finger proteins are important transcription factors in plants, responding to adversity and regulating the growth and development of plants. However, the roles of the BBX gene family of zinc-finger proteins in wintersweet (Chimonanthus praecox) have yet to be elucidated. In this study, a group IV subfamily BBX gene, CpBBX19, was identified and isolated from wintersweet. Quantitative real-time PCR (qRT-PCR) analyses revealed that CpBBX19 was expressed in all tissues and that expression was highest in cotyledons and inner petals. CpBBX19 was also expressed in all flower development stages, with the highest expression detected in early initiating bloom, followed by late initiating bloom and bloom. In addition, the expression of CpBBX19 was induced by different abiotic stress (cold, heat, NaCl, and drought) and hormone (ABA and MeJA) treatments. Heterologous expression of CpBBX19 in Arabidopsis thaliana (Arabidopsis) enhanced the tolerance of this plant to salt and drought stress as electrolyte leakage and malondialdehyde (MDA) concentrations in transgenic Arabidopsis after stress treatments were significantly lower than those in wild-type (WT) plants. In conclusion, this research demonstrated that CpBBX19 plays a role in the abiotic stress tolerance of wintersweet. These findings lay a foundation for future studies on the BBX gene family of wintersweet and enrich understanding of the molecular mechanism of stress resistance in wintersweet.

中文翻译：

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Chimonanthus praecox 的 B-Box 转录因子基因 CpBBX19 提高拟南芥的耐盐性和耐旱性

锌指蛋白是植物中重要的转录因子，对逆境作出反应，调节植物的生长发育。然而，锌指蛋白BBX基因家族在甜菜 ( Chimonanthus praecox ) 中的作用尚未阐明。在这项研究中，从冬梅中鉴定和分离了一个 IV 组亚家族BBX基因CpBBX19 。定量实时 PCR (qRT-PCR) 分析显示CpBBX19在所有组织中均有表达，并且在子叶和内花瓣中的表达最高。CPBBX19也在所有花发育阶段表达，在早期开花中检测到最高表达，其次是晚期开花和开花。此外，CpBBX19的表达受到不同非生物胁迫（冷、热、NaCl 和干旱）和激素（ABA 和 MeJA）处理的诱导。CpBBX19在拟南芥( Arabidopsis ) 中的异源表达增强了该植物对盐胁迫和干旱胁迫的耐受性，因为胁迫处理后转基因拟南芥中的电解质泄漏和丙二醛 (MDA) 浓度显着低于野生型 (WT) 植物。总之，本研究表明CpBBX19在甜菜的非生物胁迫耐受中发挥作用。这些发现为今后进一步研究梅花BBX基因家族奠定了基础，丰富了梅花抗逆分子机制的认识。