Cationic anticancer peptides have exhibited potent anti-proliferative and anti-inflammatory effects in neoplastic illness conditions. LyeTx I-b is a synthetic peptide derived from Lycosa erythrognatha spider venom that previously showed antibiotic activity in vitro and in vivo. This study focused on the effects of LyeTxI-b on a 4T1 mouse mammary carcinoma model. Mice with a palpable tumor in the left flank were subcutaneously or intratumorally injected with LyeTx I-b (5 mg/kg), which significantly decreased the tumor volume and metastatic nodules. Histological analyses showed a large necrotic area in treated primary tumors compared to the control. LyeTxI-b reduced tumor growth and lung metastasis in the 4T1 mouse mammary carcinoma model with no signs of toxicity in healthy or cancerous mice. The mechanism of action of LyeTx I-b on the 4T1 mouse mammary carcinoma model was evaluated in vitro and is associated with induction of apoptosis and cell proliferation inhibition. Furthermore, LyeTx I-b seems to be an efficient regulator of the 4T1 tumor microenvironment by modulating several cytokines, such as TGF-β, TNF-α, IL-1β, IL-6, and IL-10, in primary tumor and lung, spleen, and brain. LyeTx I-b also plays a role in leukocytes rolling and adhesion into spinal cord microcirculation and in the number of circulating leukocytes. These data suggest a potent antineoplastic efficacy ofLyeTx I-b.

中文翻译：

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LyeTx Ib 肽减轻小鼠 4T1 乳腺癌模型中的肿瘤负担和转移


阳离子抗癌肽在肿瘤疾病中表现出有效的抗增殖和抗炎作用。 LyeTx Ib 是一种源自红颌狼蛛毒液的合成肽，此前在体外和体内均显示出抗生素活性。本研究重点关注 LyeTxI-b 对 4T1 小鼠乳腺癌模型的影响。左胁腹可触及肿瘤的小鼠皮下或瘤内注射LyeTx Ib (5 mg/kg)，可显着缩小肿瘤体积和转移结节。组织学分析显示，与对照相比，治疗后的原发性肿瘤有较大的坏死区域。 LyeTxI-b 可减少 4T1 小鼠乳腺癌模型中的肿瘤生长和肺转移，而在健康或癌性小鼠中没有任何毒性迹象。 LyeTx Ib 对 4T1 小鼠乳腺癌模型的作用机制在体外进行了评估，其与诱导细胞凋亡和抑制细胞增殖有关。此外，LyeTx Ib 似乎通过调节原发性肿瘤和肺、脾中的多种细胞因子（例如 TGF-β、TNF-α、IL-1β、IL-6 和 IL-10）来有效调节 4T1 肿瘤微环境。和大脑。 LyeTx Ib 还在白细胞滚动和粘附到脊髓微循环中以及循环白细胞的数量中发挥作用。这些数据表明 LyeTx Ib 具有有效的抗肿瘤功效。