Sample-to-answer detection of a viral infectious disease is becoming increasingly important to prevent pandemic outbreaks. However, only a few microfluidic chips that use nucleic acid isolation and detection have implemented all-in-one assays in a simple and effective platform. Here, we present a microfluidic platform that isolates and detects viral RNA of influenza A (H1N1) virus from clinical swab samples in a fully automated, effective, and highly sensitive manner. The device with a vertically movable top layer and a rotationally mobile bottom layer exploits surface tension in a solution. In this way, the device effectively orchestrates multiple functions, including transporting, mixing, and washing of reagents and magnetic beads without the need for on-chip valves, pumps, and microfluidic networks that increase operational and structural complexity. With integrated heating and detection functions, the device provides fully automated sample-to-answer detection with real-time polymerase chain reaction in 60 min for positive results with high sensitivity with the limit of detection of 26 copies/reaction. Our platform is useful for accurate diagnosis of infectious diseases in point-of-care settings.

病毒传染病的样本到答案检测对于预防大流行爆发变得越来越重要。然而，只有少数使用核酸分离和检测的微流控芯片在简单有效的平台上实现了一体化检测。在这里，我们提出了一个微流体平台，该平台以全自动、有效和高度灵敏的方式从临床拭子样本中分离和检测甲型流感 (H1N1) 病毒的病毒 RNA。具有可垂直移动的顶层和可旋转移动的底层的设备利用溶液中的表面张力。通过这种方式，该设备可以有效地协调多种功能，包括试剂和磁珠的运输、混合和洗涤，而无需片上阀门、泵、以及增加操作和结构复杂性的微流体网络。该设备具有集成的加热和检测功能，可在 60 分钟内通过实时聚合酶链反应提供全自动样品到答案检测，以高灵敏度获得阳性结果，检测限为 26 个拷贝/反应。我们的平台可用于在即时护理环境中准确诊断传染病。