This paper studies the effects of hypobaric storage on the physiochemical quality of the Northland blueberry fruits, as well as their bioactive compounds, pre- and pro-antioxidant enzymes and reactive oxygen species (ROS). Different hypobaric pressures at 0.025, 0.05 and 0.075 MPa were applied to stored blueberry at 0 °C. Hypobaric treatment resulted in lower fruit decay rate compared to control, maintained respiration rate and pH of the fruit for 0.05 and 0.075 MPa treatments, which implied that fruit freshness was maintained following hypobaric storage. Also, hypobaric storage significantly retained high bioactive compounds (P < 0.05), especially total flavonol and anthocyanins after 50 days storage at 0.025 MPa compared to control. The retained bioactive compounds were reflected as high antioxidant capacity indicated by DPPH, FRAP and TEAC. The treatment 0.025MPa displayed the lowest MDA activity on day 23 and highest on day 50, which reflected delayed oxidation by hydrogen peroxide. Evidently, high levels of POD, APX, PPO and GSH antioxidant enzymes in the 0.025 MPa stored blueberries may be due to delayed ROS metabolism. Partial least square regression (PLSR) model showed that, ROS were negatively correlated with anthocyanin, POD, CAT, SSC, flavonol, TEAC, FRAP, DPPH as well as hydroxyl radical scavenging ability. Similarly, path analysis (PA) revealed that MDA was negatively correlated with TEAC, TA and fruit pH, while hydrogen peroxide was negatively correlated with GSH and protein. Of the pressures, 0.025 MPa was selected as the suitable hypobaric pressure for Northland blueberry storage as significantly retained studied indexes at relatively high values throughout 50 days storage.

本文研究了低压贮藏对北国蓝莓果实理化品质及其生物活性化合物、前抗氧化酶和促抗氧化酶以及活性氧 (ROS) 的影响。在 0°C 下对储存的蓝莓施加 0.025、0.05 和 0.075 MPa 的不同低压。与对照相比，低压处理导致较低的果实腐烂率，在 0.05 和 0.075 MPa 处理下维持了水果的呼吸速率和 pH 值，这意味着在低压储存后保持了水果的新鲜度。此外，低压储存显着保留了高生物活性化合物（P < 0.05)，尤其是与对照相比，在 0.025 MPa 下储存 50 天后的总黄酮醇和花青素。DPPH、FRAP 和 TEAC 表明保留的生物活性化合物具有高抗氧化能力。0.025MPa的处理在第23天表现出最低的MDA活性，在第50天表现出最高，这反映了过氧化氢的延迟氧化。显然，0.025 MPa 储存蓝莓中高水平的 POD、APX、PPO 和 GSH 抗氧化酶可能是由于 ROS 代谢延迟所致。偏最小二乘回归（PLSR）模型表明，ROS与花青素、POD、CAT、SSC、黄酮醇、TEAC、FRAP、DPPH以及清除羟基自由基能力呈负相关。同样，路径分析（PA）显示 MDA 与 TEAC、TA 和果实 pH 呈负相关，而过氧化氢与谷胱甘肽和蛋白质呈负相关。在压力中，选择 0.025 MPa 作为 Northland 蓝莓储存的合适低压，因为在整个 50 天的储存期间，研究指标显着保持在相对较高的值。