Medicinal plants have significant contribution in pharmaceutical industries being producers of compounds utilized as precursors for drug development. A plant of Lamiaceae family; Pseudocaryopteris foetida had not been investigated for its biomedical potential. Current research was aimed to investigate phytochemical analysis, cytotoxic potential and antioxidant activity of crude methanolic extract and fractions of Pseudocaryopteris foetida (leaves). The preliminary phytochemical analysis of crude methanolic extracts and fractions of Pseudocaryopteris foetida revealed that plant is rich in phenolic and flavonoid classes of secondary metabolites while presence of tannin was observed only in crude methanolic extract. The cytotoxicity was determined using brine shrimp lethality test. Different concentrations (25, 50, 100, 150, 200 and 250 µg/mL) of crude methanolic extract and fractions exhibited dose dependent cytotoxicity. However, The LD₅₀ for all the extracts was more than 200 µg/mL indicating weak cytotoxic potential of Pseudocaryopteris foetida. The antioxidant capabilities of crude methanolic extract and fraction of Pseudocaryopteris foetida were analyzed by in vitro bio assays including DPPH, ABTS, Reducing power and phosphomolybdate antioxidant assays using ascorbic acid as standard. The crude methanolic extract showed IC₅₀ (256.38 ± 0.6 and 314.95 ± 1.1 µg/mL) for DPPH and ABTS respectively, while total antioxidant capacity was calculated as 55.79 ± 0.5 µg/mL for crude methanolic extract of Pseudocaryopteris foetida while ascorbic acid indicated total antioxidant capacity of 71.89 ± 2.3 µg/mL. Study concluded that leaves of Pseudocaryopteris foetida were the rich source of antioxidant phytochemicals. Based on preliminary investigations further research should be focused to isolate bioactive phytochemicals as leading source of clinical medicines in future.

药用植物作为药物开发前体的化合物的生产商，对制药工业有重大贡献。唇形科植物；Pseudocaryopteris foetida的生物医学潜力尚未被研究。目前的研究旨在研究粗甲醇提取物和Pseudocaryopteris foetida（叶子）的植物化学分析、细胞毒性潜力和抗氧化活性。Pseudocaryopteris foetida粗甲醇提取物和馏分的初步植物化学分析揭示植物富含酚类和类黄酮类的次级代谢物，而仅在粗甲醇提取物中观察到单宁的存在。使用盐水虾致死试验确定细胞毒性。不同浓度（25、50、100、150、200 和 250 µg/mL）的粗甲醇提取物和级分表现出剂量依赖性细胞毒性。然而，所有提取物的 LD ₅₀均超过 200 µg/mL，表明Pseudocaryopteris foetida 的细胞毒性较弱。粗甲醇提取物和伪足蚧馏分的抗氧化能力通过体外生物测定分析，包括DPPH、ABTS、还原能力和使用抗坏血酸作为标准的磷钼酸盐抗氧化剂测定。粗甲醇提取物对 DPPH 和 ABTS 的 IC值分别为_{50 (256.38 ± 0.6 和 314.95 ± 1.1 µg/mL)，而对}Pseudocaryopteris foetida粗甲醇提取物的总抗氧化能力计算为 55.79 ± 0.5 µg/mL，而抗坏血酸指示总抗氧化能力抗氧化能力为 71.89 ± 2.3 µg/mL。研究得出结论，Pseudocaryopteris foetida的叶子是抗氧化植物化学物质的丰富来源。在初步调查的基础上，进一步的研究应集中在分离生物活性植物化学物质作为未来临床药物的主要来源。