Abstract

The supercritical fluid chromatography technique is one of the most promising and innovative technologies used in the analysis as well as the separation of various racemates. The various enantiomers have been separated using SFC due to its several merits over conventional HPLC methods such as low cost, wide polarity compatibility, higher column efficiency, and fast analyzing capability, which are desirable features for the pharmaceutical industry. Therefore, it was considered worthwhile to review the applications of SFC in achiral and chiral separations. This review encompasses the latest literature exploration of various practical aspects of SFC for enantiomeric separation, purification, and applications to the pharmaceutical industry. A detailed discussion of SFC was made in terms of productivity, stacked injections, sample solubility, particle size, green incentive, organic solvent, recycle solvents, and economical aspects. Besides, future perspectives of SFC have also been highlighted. A comparison was also made between SFC and HPLC. SFC has been found better technique than HPLC for the chiral separations on a preparative scale. This article will be highly useful for chiral chromatography in pharmaceutical industries and research laboratories.

摘要

超临界流体色谱技术是用于分析和分离各种外消旋体的最有前途和创新的技术之一。由于 SFC 与传统 HPLC 方法相比具有成本低、极性兼容性广、柱效高和快速分析能力等多项优点，因此已使用 SFC 分离各种对映异构体，这些都是制药行业的理想特性。因此，有必要回顾 SFC 在非手性和手性分离中的应用。本综述涵盖了 SFC 在对映异构体分离、纯化和制药行业应用等各个实际方面的最新文献探索。从生产率、堆叠进样、样品溶解度、粒度、绿色激励、有机溶剂、循环溶剂和经济方面。此外，还强调了证监会的未来前景。还对 SFC 和 HPLC 进行了比较。在制备规模的手性分离方面，已发现 SFC 比 HPLC 更好的技术。本文对制药行业和研究实验室的手性色谱非常有用。