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Study on the diversity of epiphytic bacteria on corn and alfalfa using Illumina MiSeq/NovaSeq high-throughput sequencing system
Annals of Microbiology ( IF 3.0 ) Pub Date : 2021-09-20 , DOI: 10.1186/s13213-021-01649-1 Meixiao Wu 1, 2 , Yuehua Wang 1, 2 , Yijing Wang 1, 2 , Guixia Liu 1, 2 , Hui Tang 1, 2 , Xuefei Wang 3 , Ming Yu 3
Annals of Microbiology ( IF 3.0 ) Pub Date : 2021-09-20 , DOI: 10.1186/s13213-021-01649-1 Meixiao Wu 1, 2 , Yuehua Wang 1, 2 , Yijing Wang 1, 2 , Guixia Liu 1, 2 , Hui Tang 1, 2 , Xuefei Wang 3 , Ming Yu 3
Affiliation
To investigate the diversity of the epiphytic bacteria on corn (Zea mays) and alfalfa (Medicago sativa) collected in Hengshui City and Xingtai City, Hebei Province, China, and explore crops suitable for natural silage. The Illumina MiSeq/NovaSeq high-throughput sequencing system was used to conduct paired-end sequencing of the community DNA fragments from the surface of corn and alfalfa collected in Hengshui and Xingtai. QIIME2 and R software were used to sort and calculate the number of sequences and taxonomic units for each sample. Thereafter, the alpha and beta diversity indices at of species level were calculated, and the abundance and distribution of taxa were analyzed and compared between samples. At phylum level, the dominant groups were Proteobacteria (70%), Firmicutes (13%), Actinobacteria (9%), and Bacteroidetes (7%). Meanwhile, the dominant genera were Pseudomonas (8%), Acinetobacter (4%), Chryseobacterium (3%), and Hymenobacter (1%). Enterobacteriaceae (24%) were the most predominant bacteria in both the corn and alfalfa samples. Alpha diversity analysis and beta diversity indices revealed that the diversity of epiphytic microbial communities was significantly affected by plant species but not by region. The diversity and richness of the epiphytic bacterial community of alfalfa were significantly higher than those of corn. This study contributes to the expanding knowledge on the diversity of epiphytic bacteria in corn and alfalfa silage and provides a basis for the selection of raw materials.
更新日期：2021-09-21
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