Lignocellulose, derived from non-food crops, is a competitive renewable sugar source. In this study, it was pretreated to produce lignocellulose hydrolysate (reducing sugar), and then used as co-substrate to accumulate polyhydroxyalkanoate (PHA) with its fermented product volatile fatty acids (VFAs). The rule of PHA accumulation and structural characterization from single VFAs, lignocellulose hydrolysate and VFAs co-substrate two carbon sources were investigated. As a functional green material, it is expected to replace the traditional petroleum-based polymer. During the culture process, compared with single VFAs carbon source, higher PHA production of 3049 mg COD/L was achieved with the addition of co-substrate, especially the proportion of 3-hydroxyvalerate (HV) monomer reached 44.9 % at C/N ratio of 33. The average molecular weight of PHA was 1.16∼1.27 × 10⁵ Da, with a polydispersity index of 2.08∼2.46. The synthesized PHA was thermal stable up to 285 °C, which was 14 °C higher than that of commercial poly (hydroxybutyrate-co-hydroxyvalerate) (PHBV). As a biopolymer film, it possessed high tensile strength (8.13 ± 0.607 MPa), Young's modulus (875.47 ± 26.21 MPa).

源自非粮食作物的木质纤维素是一种具有竞争力的可再生糖源。在这项研究中，它被预处理产生木质纤维素水解物（还原糖），然后作为共底物积累聚羟基链烷酸酯（PHA）及其发酵产物挥发性脂肪酸（VFA）。研究了单一 VFA、木质纤维素水解物和 VFA 共底物两种碳源的 PHA 积累规律和结构表征。作为一种功能性绿色材料，有望取代传统的石油基聚合物。在培养过程中，与单一VFAs碳源相比，加入共底物后PHA产量更高，达到3049 mg COD/L，尤其是3-羟基戊酸（HV）单体在C/N比下的比例达到44.9% 33。PHA的平均分子量是1。⁵ Da，多分散指数为2.08∼2.46。合成的 PHA 在高达 285°C 的温度下具有热稳定性，比商业聚（羟基丁酸-共-羟基戊酸酯）（PHBV）的热稳定性高 14°C。作为生物聚合物薄膜，它具有高拉伸强度（8.13 ± 0.607 MPa）和杨氏模量（875.47 ± 26.21 MPa）。