Factors limiting the production of the greenhouse gases nitrous oxide (N₂O) and carbon dioxide (CO₂) were investigated in three incubation experiments conducted with soil from top- and subsoil horizons of a peatland which had an acid sulphate mineral subsoil derived from black schists. The effect of moisture was investigated by equilibrating undisturbed soil samples from three horizons (H2, Cg and Cr) at −10, −60 or −100 cm matric potential and measuring the gas production. In the second experiment, the effects of temperature and various substrates were studied by incubating disturbed soil samples in aerobic conditions at 5 or 20 °C, and measuring basal respiration and N₂O production before and after adding water, glucose or ammonium into the soil. In the third experiment, the effects of added glucose and/or nitrate on the denitrification in soil samples from four horizons (H1, H2, Cg and Cr were investigated by acetylene inhibition and monitoring of N₂O production during a 48-h anaerobic incubation. The production of CO₂ in the topmost peat horizon was largest at −10 cm matric potential, and it was larger than those in the mineral subsoil also at −60 and −100 cm potentials. In contrast, drainage seemed to increase N₂O production, whereas in the wettest condition the production of N₂O in the mineral subsoil was small and the peat horizon was a sink of N₂O. Lowering of temperature (from 20 °C to 5 °C) decreased CO₂ production, as expected, but it had almost no role in the production of N₂O in aerobic conditions. Glucose addition increased the aerobic production of CO₂ in peat, but it had a minor effect in the mineral horizons. Lack of C source (glucose) was limiting anaerobic N₂O production in the uppermost peat horizon, while in all other horizons, nitrate proved to be the most limiting factor. It is concluded that peatlands with black schist derived acid sulphate subsoil horizons, such as in this study, have high microbial activity in the peaty topsoil horizons but little microbial activity in the mineral subsoil. These findings are contrary to previous results obtained in sediment-derived acid sulphate soils.

在三个孵化实验中研究了限制温室气体一氧化二氮 (N ₂ O) 和二氧化碳 (CO ₂ ) 产生的因素，这些实验是用泥炭地表层和底土层的土壤进行的，泥炭地具有源自黑色的酸性硫酸盐矿物底土片岩。通过在 -10、-60 或 -100 cm 基质势下平衡来自三个层位（H2、Cg 和 Cr）的未受干扰土壤样品并测量气体产量来研究水分的影响。在第二个实验中，通过在 5 或 20 °C 的有氧条件下培养扰动的土壤样品，并测量基础呼吸和 N ₂来研究温度和各种基质的影响。向土壤中加入水、葡萄糖或铵前后的 O 产量。在第三个实验中，添加葡萄糖和/或硝酸盐对来自四个层级（H1、H2、Cg 和 Cr）的土壤样品的反硝化作用通过乙炔抑制和在 48 小时厌氧培养期间监测 N ₂ O 的产生进行研究.最顶层泥炭层的CO ₂产量在-10 cm 基质势最大，并且在-60 和-100 cm 势能下也大于矿物底土中的CO ₂。相反，排水似乎增加了N ₂ O产量，而在最潮湿的条件下，矿物底土中 N ₂ O的产量很小，泥炭层是 N ₂的汇O.正如预期的那样，降低温度（从 20 °C 到 5 °C）降低了 CO _{2 的}产生，但它在有氧条件下几乎没有产生 N ₂ O 的作用。添加葡萄糖增加了泥炭中CO ₂的有氧产量，但对矿物层的影响较小。缺乏 C 源（葡萄糖）限制了厌氧 N ₂最上层泥炭层中的 O 产量，而在所有其他层中，硝酸盐被证明是最大的限制因素。得出的结论是，具有黑色片岩衍生的酸性硫酸盐底土层的泥炭地，例如在本研究中，在泥炭表土层中具有高微生物活性，但在矿物底土中微生物活动很少。这些发现与先前在沉积物衍生的酸性硫酸盐土壤中获得的结果相反。