Rational programming of double network hydrogel (DN gel) structure is an effective strategy to acquire satisfied mechanical properties and innovative functions. Herein, we report a novel electrochemically strategy for rational design of a DN gel from the blends of two stimuli-responsive self-assembling polysaccharides chitosan (CS, pH-responsive) and agarose (Agar, thermally responsive) by using externally applied electrical signal/temperature stimulations to cue sequential self-assemblies. Specifically, considering of the remarkable difference in the filed-induced chains' migration behavior at liquid/solid systems, chitosan's electrophoretic deposition (EDP) processes were performed comparably at different temperatures and two types of DN gels were fabricated with dramatically different growing kinetics, tailored crystalline structures and enhanced mechanical properties. Moreover, the bioinspired multilayer structure could be facially imparted in the DN gels by using an oscillating electrical signal, and their great potential applications such as the pH/ethanol-sensitive device or versatile platform for staged drug delivery have been revealed respectively. Eventually, we demonstrated that the electrical signal is highly advantageous in preparing DN gels that able to: (i) provide complex pH cues to guide polymer chains self-assembled with specific patterning structures; (ii) quantitative analysis of the gelation process via transfer charges; (iii) offer great possibilities for hydrogel construction via electric filed-induced chain migration. Therefore, we believe this electro-biochemically synthesizing approach could spur new insight into the multifunctional hydrogel materials constructions and biomedical related applications.

双网络水凝胶（DN凝胶）结构的合理规划是获得满意的力学性能和创新功能的有效策略。在此，我们报告了一种新的电化学策略，通过使用外部施加的电信号，从两种刺激响应性自组装多糖壳聚糖（CS，pH 响应性）和琼脂糖（琼脂，热响应性）的混合物中合理设计 DN 凝胶/温度刺激以提示顺序自组装。具体而言，考虑到液/固系统中场致链迁移行为的显着差异，壳聚糖的电泳沉积 (EDP) 工艺在不同温度下进行比较，并且制备的两种 DN 凝胶具有显着不同的生长动力学，定制的晶体结构和增强的机械性能。此外，生物启发的多层结构可以通过使用振荡电信号在 DN 凝胶中进行面部传递，并且它们的巨大潜在应用，例如 pH/乙醇敏感装置或用于分阶段给药的多功能平台已分别被揭示。最终，我们证明了电信号在制备 DN 凝胶方面非常有利，它能够：(i) 提供复杂的 pH 提示，以引导具有特定图案结构的聚合物链自组装；(ii) 通过转移电荷对凝胶化过程进行定量分析；(iii) 通过电场诱导的链迁移为水凝胶构建提供了巨大的可能性。所以，