Cerebellar outputs take polysynaptic routes to reach the rest of the brain, impeding conventional tracing. Here, we quantify pathways between the cerebellum and forebrain by using transsynaptic tracing viruses and a whole-brain analysis pipeline. With retrograde tracing, we find that most descending paths originate from the somatomotor cortex. Anterograde tracing of ascending paths encompasses most thalamic nuclei, especially ventral posteromedial, lateral posterior, mediodorsal, and reticular nuclei. In the neocortex, sensorimotor regions contain the most labeled neurons, but we find higher densities in associative areas, including orbital, anterior cingulate, prelimbic, and infralimbic cortex. Patterns of ascending expression correlate with c-Fos expression after optogenetic inhibition of Purkinje cells. Our results reveal homologous networks linking single areas of the cerebellar cortex to diverse forebrain targets. We conclude that shared areas of the cerebellum are positioned to provide sensory-motor information to regions implicated in both movement and nonmotor function.

小脑输出通过多突触途径到达大脑的其他部分，阻碍了传统的追踪。在这里，我们通过使用跨突触追踪病毒和全脑分析管道来量化小脑和前脑之间的通路。通过逆行追踪，我们发现大多数下行路径起源于躯体运动皮层。上行路径的顺行追踪包括大多数丘脑核，尤其是腹侧后内侧、外侧后侧、中背侧和网状核。在新皮质中，感觉运动区域包含标记最多的神经元，但我们发现关联区域的密度更高，包括眼眶、前扣带回、前肢和下肢皮质。在浦肯野细胞的光遗传学抑制后，上升表达模式与 c-Fos 表达相关。我们的研究结果揭示了将小脑皮层的单个区域与不同的前脑目标联系起来的同源网络。我们得出结论，小脑的共享区域定位为向涉及运动和非运动功能的区域提供感觉运动信息。