Background

lncRNAs are modulatory factors with critical function in the tumorigenesis pathways, introducing them as promising therapeutic and diagnostic biomarkers for different cancers. This study is thus aimed to evaluate the differences in PVT1 and ZFAS1 gene expression in tumorous tissues as compared with adjacent healthy non-tumorous biopsies of gastric cancer cases.

Methods

One hundred two pairs of tumorous and adjacent non-tumorous biopsies of GC cases were sampled. RNA isolation and cDNA production were carried out. The qRT-PCR was performed to evaluate the expression of PVT1 and ZFAS1 genes. Moreover, the associations between PVT1 or ZFAS1 and clinicopathological characteristics as well as the biomarker roles of the lncRNAs were assessed.

Results

The PVT1 and ZFAS1 expressions showed a significant increase and decrease in GC samples as compared with non-cancerous tissues, respectively. PVT1 expression was significantly associated with and lymph-node involvement (p = 0.0007). Moreover, ZFAS1 expression demonstrated a significant association with lymph-node involvement (p = 0.0005), and tumor size >5 cm (p = 0.003). The findings of the ROC curve revealed that PVT1 and ZFAS1 may act as a possible biomarker with AUC of 0.71 and 0.79, specificity of 78.43% and 79.41%, and sensitivity of 55.88% and 64.71%.

Conclusions

Regarding upregulation of PVT1 and downregulation of ZFAS1 in human GC samples, these genes may respectively act as oncogenic and tumor-suppressive factors in GC cases. Furthermore, PVT1 and ZFAS1 can be considered as possible biomarkers for the detection and treatment of GC cases.

中文翻译：

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PVT1和ZFAS1 lncRNAs在伊朗人群胃癌组织取样中的表达及其生物标志物价值

背景

lncRNA是在肿瘤发生途径中具有关键功能的调节因子，将它们作为不同癌症的有希望的治疗和诊断生物标志物。因此，本研究旨在评估肿瘤组织中 PVT1 和 ZFAS1 基因表达与胃癌病例相邻健康非肿瘤活检相比的差异。

方法

采集了 102 对 GC 病例的肿瘤和相邻非肿瘤活检样本。进行了RNA分离和cDNA生产。进行 qRT-PCR 以评估 PVT1 和 ZFAS1 基因的表达。此外，还评估了 PVT1 或 ZFAS1 与临床病理学特征以及 lncRNA 的生物标志物作用之间的关联。

结果

与非癌组织相比，GC 样品中 PVT1 和 ZFAS1 的表达分别显着增加和减少。PVT1 表达与淋巴结受累显着相关（p  = 0.0007）。此外，ZFAS1 表达与淋巴结受累（p  = 0.0005）和肿瘤大小> 5 cm（p  = 0.003）显着相关。ROC曲线的结果显示，PVT1和ZFAS1可能作为一种可能的生物标志物，其AUC分别为0.71和0.79，特异性分别为78.43%和79.41%，敏感性分别为55.88%和64.71%。

结论

关于人类 GC 样本中 PVT1 的上调和 ZFAS1 的下调，这些基因可能分别在 GC 病例中充当致癌和肿瘤抑制因子。此外，PVT1 和 ZFAS1 可被视为检测和治疗 GC 病例的可能生物标志物。