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The role of temperature in the formation of human–mimetic artificial cell membranes using droplet interface bilayers (DIBs)
Soft Matter ( IF 2.9 ) Pub Date : 2021-09-08 , DOI: 10.1039/d1sm00668a
Jaime L Korner 1 , Katherine S Elvira 1
Affiliation  

Droplet interface bilayers (DIBs) have recently started to be used as human–mimetic artificial cell membranes. DIBs are bilayer sections created at the interface of two aqueous droplets, such that one droplet can be used as a donor compartment and the other as an acceptor compartment for the quantification of molecular transport across the artificial cell membrane. However, synthetic phospholipids are overwhelmingly used to create DIBs instead of naturally derived phospholipids, even though the diverse distribution of phospholipids in the latter is more biomimetic. We present the first systematic study of the role of temperature in DIB formation, which shows that the temperature at which DIBs are formed is a key parameter for the formation of DIBs using naturally derived phospholipids in a microfluidic platform. The phospholipids that are most abundant in mammalian cell membranes (phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), and phosphatidylinositol (PI)) only form DIBs when the temperature is above the phase transition temperature (Tm). Similarly, DIB formation usually only occurs above the highest Tm of a single phospholipid in a bespoke formulation. In addition, we show a new phenomenon wherein the DIB “melts” without disintegrating for bilayers formed predominantly of phospholipids that occupy cylindrical spaces. We also demonstrate differences in DIB formation rates as well as permeability of these biomimetic membranes. Given the difficulties associated with making DIBs using naturally derived phospholipids, we anticipate this work will illuminate the role of phospholipid phase transition in mono- and bilayer formation and lay the foundation for DIBs to be used as human–mimetic artificial cell membranes.

中文翻译:

温度在使用液滴界面双层 (DIB) 形成拟人人工细胞膜中的作用

液滴界面双层(DIB)最近开始用作模拟人的人工细胞膜。DIB 是在两个水性液滴的界面处创建的双层部分,这样一个液滴可以用作供体隔室,另一个用作受体隔室,用于量化跨人工细胞膜的分子传输。然而,合成磷脂绝大多数用于制造 DIB,而不是天然衍生的磷脂,尽管后者中磷脂的多样化分布更具仿生性。我们首次系统研究了温度在 DIB 形成中的作用,这表明 DIB 形成的温度是在微流体平台中使用天然衍生的磷脂形成 DIB 的关键参数。Ť)。类似地,DIB形成通常只发生高于最高Ť在一个定制的制剂的单个磷脂。此外,我们展示了一种新现象,其中DIB“熔化”而不分解主要由占据圆柱形空间的磷脂形成的双层。我们还展示了这些仿生膜的 DIB 形成率和渗透性的差异。鉴于使用天然衍生的磷脂制造 DIB 存在困难,我们预计这项工作将阐明磷脂相变在单层和双层形成中的作用,并为 DIB 用作模拟人的人工细胞膜奠定基础。
更新日期:2021-09-21
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