ABSTRACT

Spent coffee ground (SCG) is a residue released during coffee brewing. Disposal of SCG is able to lead to a loss of bioactive constituents and have a negative impact upon the environment. This study aimed to explore fatty acids, bioactive phenolics, antioxidant and anti-aging potentials of SCG samples obtained from coffee shops in Ho Chi Minh city, Vietnam. The oil content of the SCG samples ranged from 6.3 to 10.5%, and linoleic (43%), palmitic (33.4%) and oleic (11%) acids were identified as major constituents of the oil. Chlorogenic acid was the most abundant phenolic acid examined (832.9–7657.7 μg/g of dry weight), followed by ferulic acid (40.5–1041.7 μg/g). Through anti-collagenase and anti-elastase assays, SCG showed a promising anti-aging activity, with IC₅₀ values comparable with those of epigallocatechin gallate. The correlation analysis revealed gallic acid positively correlated with the free radical scavenging potential evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and the inhibitory effect on elastase. The results demonstrated that utilization of SCG prepared from Vietnamese coffee could open new avenues for food and cosmeceutical industry.

摘要

废咖啡渣 (SCG) 是咖啡冲煮过程中释放的残留物。SCG 的处置会导致生物活性成分的损失并对环境产生负面影响。本研究旨在探索从越南胡志明市咖啡店获得的 SCG 样品的脂肪酸、生物活性酚类物质、抗氧化和抗衰老潜力。SCG 样品的油含量范围为 6.3% 至 10.5%，亚油酸 (43%)、棕榈酸 (33.4%) 和油酸 (11%) 被确定为油的主要成分。绿原酸是检测到的最丰富的酚酸（832.9–7657.7 μg/g 干重），其次是阿魏酸（40.5–1041.7 μg/g）。通过抗胶原酶和抗弹性蛋白酶检测，SCG 显示出有希望的抗衰老活性，IC ₅₀值与表没食子儿茶素没食子酸酯相当。相关分析表明没食子酸与DPPH（2,2-二苯基-1-苦基肼）测定评估的自由基清除能力和对弹性蛋白酶的抑制作用呈正相关。结果表明，利用从越南咖啡制备的 SCG 可以为食品和药妆行业开辟新的途径。