Carbon fiber microelectrode arrays based on diazonium salt and single-walled carbon nanotubes composites (DS-SWCNT/CFMEA) have been fabricated, and it developed for the simultaneous monitoring of dopamine (DA) and serotonin (5-HT) with differential pulse voltammary (DPV). The diazonium salt can improve the water-solubility of single-walled carbon nanotubes and show good selectivity to DA, thus DS-SWCNT/CFMEA exhibits enhanced electrocatalytic activity for the oxidation of DA and 5-HT, and well antifouling ability to the other biomolecules. Moreover, DS-SWCNT/CFMEA shows the wider liner range, and the good performance of precision, reproducibility and biocompatibility. The excellent characteristics of the prepared microsensor array make it to be used to monitor the release of DA and 5-HT in the mouse brain striatum of different group over time. Meanwhile, the results of in vivo on line assay further confirmed the pharmacological effects of Uncaria alkaloid extract solution on DA and 5-HT. This research may provide a new method for monitoring the release of neurobiomolecules, and the microsensor array are expected to be a tool for the study of pharmacological and physiological processes on line in vivo.

制备了基于重氮盐和单壁碳纳米管复合材料（DS-SWCNT/CFMEA）的碳纤维微电极阵列，并开发用于差分脉冲伏安法同时监测多巴胺（DA）和血清素（5-HT）。 DPV）。重氮盐可以提高单壁碳纳米管的水溶性并对DA表现出良好的选择性，因此DS-SWCNT/CFMEA对DA和5-HT的氧化具有增强的电催化活性，对其他生物分子具有良好的防污能力. 此外，DS-SWCNT/CFMEA 显示出更宽的衬管范围，以及良好的精度、重现性和生物相容性。制备的微传感器阵列的优良特性使其可用于监测不同组小鼠脑纹状体中 DA 和 5-HT 随时间的释放。钩藤生物碱提取液对 DA 和 5-HT。该研究可能为监测神经生物分子的释放提供一种新方法，微传感器阵列有望成为体内在线药理和生理过程研究的工具。