Synthesis and application of smart gel material modified silica microspheres for pH-responsive hydrophilicity in liquid chromatography,Analyst

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Synthesis and application of smart gel material modified silica microspheres for pH-responsive hydrophilicity in liquid chromatography
Analyst ( IF 3.6 ) Pub Date : 2021-09-21 , DOI: 10.1039/d1an01182k
Fangbin Fan _{1,

2} , Licheng Wang ₁ , Xiaofeng Lu ₁ , Xiaojing Liang ₁ , Yong Guo ₁

Affiliation

Inspired by porous smart gel materials, we designed pH-responsive polymer-modified silica microspheres as liquid chromatography stationary phase materials by a one-step strategy. The free radicals generated by the oxidation of dopamine are used to initiate the cross-linking polymerization of functional monomers. At the same time, the good adhesion of dopamine enables the polymer to be modified on silica. The hydrophilicity of this new stationary phase can change in response to the pH of the mobile phase and the stationary phase has weaker hydrophilicity under acidic (pH = 3.78) mobile phase conditions and stronger hydrophilicity under neutral mobile phase conditions. The hydrophilicity difference of the stationary phase leads to the selectivity difference in separation. To evaluate the chromatographic performance of this new stationary phase, 10 oligosaccharides and 9 nucleosides/bases were separated on this stationary phase. This paper will provide good guidance for us to achieve more pH-responsive hydrophilic/hydrophobic stationary phases in the future.

中文翻译：

液相色谱pH响应亲水性智能凝胶材料改性硅胶微球的合成及应用

受多孔智能凝胶材料的启发，我们通过一步策略设计了 pH 响应性聚合物改性二氧化硅微球作为液相色谱固定相材料。多巴胺氧化产生的自由基用于引发功能单体的交联聚合。同时，多巴胺的良好附着力使聚合物能够在二氧化硅上进行改性。这种新型固定相的亲水性会随着流动相的pH值而变化，在酸性（pH=3.78）流动相条件下，固定相亲水性较弱，在中性流动相条件下亲水性较强。固定相的亲水性差异导致分离的选择性差异。为了评估这种新型固定相的色谱性能，在该固定相上分离了 10 种寡糖和 9 种核苷/碱基。本文将为我们在未来实现更多的pH响应性亲水/疏水固定相提供很好的指导。

更新日期：2021-09-21

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