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Engineering high coenzyme Q10 tomato
Metabolic Engineering ( IF 6.8 ) Pub Date : 2021-09-21 , DOI: 10.1016/j.ymben.2021.09.007
Hang Fan 1 , Yan Liu 2 , Chen-Yi Li 3 , Yan Jiang 4 , Jiao-Jiao Song 2 , Lei Yang 5 , Qing Zhao 1 , Yong-Hong Hu 5 , Xiao-Ya Chen 1 , Jing-Jing Xu 5
Affiliation  

Coenzyme Q (CoQ) is vital for energy metabolism in living organisms. In humans, CoQ10 deficiency causes diseases and must be replenished via diet; however, CoQ content in plant foods is primarily low. Here, we report the breeding of high CoQ10 tomato lines by expressing four enzymes with a fruit-specific promoter, which modifies the chloroplast chorismate pathway, enhances cytosolic isoprenoid biosynthesis, and up-regulates the first two reactions in mitochondrion that construct the CoQ10 polyisoprenoid tail. We show that, while the level of the aromatic precursor could be markedly elevated, head group prenylation is the key to increasing the final CoQ10 yield. In the HUCD lines expressing all four transgenes, the highest CoQ10 content (0.15 mg/g dry weight) shows a seven-fold increase from the wild-type level and reaches an extraordinarily rich CoQ10 food grade. Overviewing the changes in other terpenoids by transcriptome and metabolic analyses reveals variable contents of carotenoids and α-tocopherol in the HUCD lines. In addition to the enigmatic relations among different terpenoid pathways, high CoQ10 plants maintaining substantial levels of either vitamin can be selected. Our investigation paves the way for the development of CoQ10-enriched crops as dietary supplements.



中文翻译:

工程高辅酶Q10番茄

辅酶 Q (CoQ) 对生物体的能量代谢至关重要。在人类中,CoQ 10缺乏会导致疾病,必须通过饮食补充;然而,植物性食品中的辅酶Q含量主要较低。在这里,我们报告了通过表达四种带有水果特异性启动子的酶来培育高 CoQ 10番茄品系,该启动子修饰叶绿体分支酸途径,增强细胞质类异戊二烯生物合成,并上调构建 CoQ 10 的线粒体中的前两个反应聚异戊二烯尾。我们表明,虽然芳香族前体的水平可以显着提高,但头基异戊二烯化是提高最终 CoQ 10产量的关键。在表达所有四种转基因的 HUCD 系中,最高的 CoQ10 的含量(0.15 毫克/克干重)比野生型水平增加了七倍,达到了非常丰富的 CoQ 10食品级。通过转录组和代谢分析概述其他萜类化合物的变化,揭示了 HUCD 系中类胡萝卜素和 α-生育酚的可变含量。除了不同萜类化合物途径之间的神秘关系外,还可以选择高 CoQ 10植物,并保持大量维生素。我们的研究为开发富含 CoQ 10 的作物作为膳食补充剂铺平了道路。

更新日期:2021-09-24
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