A new type of polyoxyphenylpropeone fluorescent probe was designed by one pot method. In this article, through a simple synthetic route, a sensitive fluorescent probe for the detection of Fe³⁺ and Cu²⁺ in vitro was successfully synthesized. Interestingly, the fluorescence of this novel probe quenched significantly in the presence of Fe³⁺ and Cu²⁺. Ketone carbonyl group and oxygen atom form the natural coordination site of iron ion and copper ion. After the coordination with metal ion, the conjugated system of the compound is broken, resulting in fluorescence quenching. At the same time, transmission electron microscope (TEM) were conducted to found its morphology property and the result shows that the polymer is stacked in layers in the images. The results of cell imaging confirmed that the polymer probe possessed favorable biocompatibility. The in vivo experiment of mice also gained the same conclusion with high penetration and fluorescence intensity. Due to the convenience of synthesis and superior recognition performance, this fluorescent probe has great potential in the study of accumulation and transformation of heavy metal ions in vivo and clinical detection of Fe³⁺ and Cu²⁺.

采用一锅法设计了一种新型聚氧苯丙酮荧光探针。本文通过简单的合成路线，成功合成了一种用于体外检测Fe ³⁺和Cu ²⁺的灵敏荧光探针。有趣的是，这种新型探针的荧光在 Fe ³⁺和 Cu ²⁺存在下显着猝灭. 酮羰基和氧原子形成铁离子和铜离子的天然配位点。与金属离子配位后，化合物的共轭体系被破坏，导致荧光猝灭。同时，通过透射电子显微镜（TEM）观察其形貌特性，结果表明聚合物在图像中呈层状堆积。细胞成像结果证实聚合物探针具有良好的生物相容性。小鼠体内实验也得到了相同的结论，具有高穿透力和荧光强度。由于合成的方便和优越的识别性能，该荧光探针在研究重金属离子在体内的积累和转化方面具有很大的潜力。Fe ³⁺和Cu ^{2+ 的}临床检测。