A slow degradation rate and low transformation efficiency are the main problems in the biodegradation of polycyclic aromatic hydrocarbons (PAHs). This study selected pyrene as the target PAH to investigate the effect of ferrous ion and ferric ion on pyrene degradation. The driving effect and mechanism, including the interaction between pyrene and iron ions and the bacterial physiological response during the biodegradation process by Rhodococcus ruber strain L9, were investigated. The results showed that iron ions did not enhance bacterial growth but improved bacteria’s pyrene removal capacity, contributing to the total efficiency of pyrene biodegradation. The process started with an initial formation of “cation-π” between Fe (III) and pyrene, which subsequently drove the pyrene removal process and accelerated the bacterial metabolic process. Moreover, a significant increase in the protein concentration, catechol dioxygenase (C12O and C23O) activities, and intracellular protein regulation in crude enzyme solution indicate a positive response of the bacteria during the iron ion-enhanced pyrene degradation process.

多环芳烃（PAHs）生物降解的主要问题是降解速度慢和转化效率低。本研究选择芘作为目标多环芳烃，研究亚铁离子和三价铁离子对芘降解的影响。红球菌生物降解过程中芘与铁离子相互作用及细菌生理反应的驱动作用与机制菌株 L9，进行了研究。结果表明，铁离子没有促进细菌生长，但提高了细菌的芘去除能力，有助于芘生物降解的总效率。该过程开始于 Fe (III) 和芘之间最初形成“阳离子-π”，随后推动芘去除过程并加速细菌代谢过程。此外，粗酶溶液中蛋白质浓度、儿茶酚双加氧酶（C12O 和 C23O）活性和细胞内蛋白质调节的显着增加表明细菌在铁离子增强芘降解过程中的积极响应。