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Hernandulcin Production in Cell Suspensions of Phyla Scaberrima: Exploring Hernandulcin Accumulation through Physical and Chemical Stimuli
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2021-09-21 , DOI: 10.1002/cbdv.202100611 Nemesio Villa-Ruano 1 , Carlos J Castro-Juárez 2 , Edmundo Lozoya-Gloria 3 , Sergio Alberto Ramírez-García 2 , Ramiro Cruz-Durán 4 , Jenaro Leocadio Varela-Caselis 5
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2021-09-21 , DOI: 10.1002/cbdv.202100611 Nemesio Villa-Ruano 1 , Carlos J Castro-Juárez 2 , Edmundo Lozoya-Gloria 3 , Sergio Alberto Ramírez-García 2 , Ramiro Cruz-Durán 4 , Jenaro Leocadio Varela-Caselis 5
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Hernandulcin (HE) is a non-caloric sweetener synthesized by the Mexican medicinal plant Phyla scaberrima. Herein we present the results of HE production through cell suspensions of P. scaberrima as well as the influence of pH, temperature, biosynthetic precursors and potential elicitors to enhance HE accumulation. The incorporation of mevalonolactone (30–400 mg L−1) farnesol (30–400 mg L−1), AgNO3 (0.025–0.175 M), cellulase (5–60 mg L−1; 0.3 units/mg), chitin (20–140 mg L−1) and (+)-epi-α-bisabolol (300-210 mg L−1) to the cell suspensions, resulted in a differential accumulation of HE and biomass. Among elicitors assayed, chitin, cellulase and farnesol increased HE production from 93.2 to ∼160 mg L−1 but, (+)-epi-α-bisabolol (obtained by a synthetic biology approach) increased HE accumulation up to 182.7 mg L−1. HE produced by the cell suspensions was evaluated against nine strains from six species of gastrointestinal bacteria revealing moderate antibacterial activity (MIC, 214–465 μg mL−1) against Staphylococcus aureus, Escherichia coli and Helicobacter pylori. Similarly, HE showed weak toxicity against Lactobacillus sp. and Bifidobacterium bifidum (>1 mg mL−1), suggesting a selective antimicrobial activity on some species of gut microbiota. According to our results, chitin and (+)-epi-α-bisabolol were the most effective molecules to enhance HE accumulation in cell suspensions of P. scaberrima.
中文翻译：
Hernandulcin 在 Phyla Scaberrima 细胞悬液中的产生：通过物理和化学刺激探索 Hernandulcin 的积累
Hernandulcin (HE) 是一种由墨西哥药用植物Phyla scaberrima合成的无热量甜味剂。在此，我们展示了通过P. scaberrima 的细胞悬浮液产生 HE 的结果，以及 pH、温度、生物合成前体和潜在诱导剂对增强 HE 积累的影响。甲瓦龙内酯 (30–400 mg L -1 )、法尼醇 (30–400 mg L -1 )、AgNO3 (0.025–0.175 M)、纤维素酶 (5–60 mg L -1 ; 0.3 units/mg)、几丁质 ( 20–140 mg L -1 ) 和 (+)- epi -α-红没药醇 (300-210 mg L -1) 到细胞悬浮液中，导致 HE 和生物量的不同积累。在检测的诱导剂中，几丁质、纤维素酶和法尼醇将 HE 产量从 93.2 增加到 160 mg L -1，但是，(+)- epi -α-红没药醇（通过合成生物学方法获得）将 HE 积累增加到 182.7 mg L -1 . 细胞悬液产生的 HE 对来自六种胃肠道细菌的九种菌株进行了评估，显示出对金黄色葡萄球菌、大肠杆菌和幽门螺杆菌的中等抗菌活性（MIC，214–465 μg mL -1）。同样，HE 对Lactobacillus sp. 的毒性较弱。和Bifidobacterium bifidum (>1 mg mL -1 )，表明对某些肠道微生物群具有选择性抗菌活性。根据我们的结果，几丁质和 (+)- epi -α-红没药醇是增强P. scaberrima细胞悬浮液中 HE 积累的最有效分子。
更新日期：2021-11-15
中文翻译：
Hernandulcin 在 Phyla Scaberrima 细胞悬液中的产生：通过物理和化学刺激探索 Hernandulcin 的积累
Hernandulcin (HE) 是一种由墨西哥药用植物Phyla scaberrima合成的无热量甜味剂。在此，我们展示了通过P. scaberrima 的细胞悬浮液产生 HE 的结果，以及 pH、温度、生物合成前体和潜在诱导剂对增强 HE 积累的影响。甲瓦龙内酯 (30–400 mg L -1 )、法尼醇 (30–400 mg L -1 )、AgNO3 (0.025–0.175 M)、纤维素酶 (5–60 mg L -1 ; 0.3 units/mg)、几丁质 ( 20–140 mg L -1 ) 和 (+)- epi -α-红没药醇 (300-210 mg L -1) 到细胞悬浮液中，导致 HE 和生物量的不同积累。在检测的诱导剂中，几丁质、纤维素酶和法尼醇将 HE 产量从 93.2 增加到 160 mg L -1，但是，(+)- epi -α-红没药醇（通过合成生物学方法获得）将 HE 积累增加到 182.7 mg L -1 . 细胞悬液产生的 HE 对来自六种胃肠道细菌的九种菌株进行了评估，显示出对金黄色葡萄球菌、大肠杆菌和幽门螺杆菌的中等抗菌活性（MIC，214–465 μg mL -1）。同样，HE 对Lactobacillus sp. 的毒性较弱。和Bifidobacterium bifidum (>1 mg mL -1 )，表明对某些肠道微生物群具有选择性抗菌活性。根据我们的结果，几丁质和 (+)- epi -α-红没药醇是增强P. scaberrima细胞悬浮液中 HE 积累的最有效分子。
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