Bioconversion of lignocellulosic biomass into value-added products relies on polysaccharides depolymerization by carbohydrate active enzymes. This work reports biochemical characterization of Paludibacter propionicigenes xylanase from GH10 (PpXyn10A) and its application for enzymatic xylooligosaccharides (XOS) production from commercial heteroxylans and liquor of hydrothermally pretreated corn cobs (PCC). PpXyn10A is tolerant to ethanol and NaCl, and releases xylobiose (X2) and xylotriose (X3) as the main hydrolytic products. The conversion rate of complex substrates into short XOS was approximately 30% for glucuronoxylan and 8.8% for rye arabinoxylan, after only 4 h; while for PCC, PpXyn10A greatly increased unbranched XOS yields. B. adolescentis fermentation with XOS from beechwood glucuronoxylan produced mainly acetic and lactic acids. Structural analysis shows that while the glycone region of PpXyn10A active site is well preserved, the aglycone region has aromatic interactions in the +2 subsite that may explain why PpXyn10A does not release xylose.

木质纤维素生物质向增值产品的生物转化依赖于碳水化合物活性酶对多糖的解聚。这项工作报告了来自 GH10 ( Pp Xyn10A) 的Paludibacter propionicigenes木聚糖酶的生化特征及其在商业杂木聚糖和水热预处理玉米芯 (PCC) 液中酶促木寡糖 (XOS) 生产中的应用。Pp Xyn10A耐乙醇和NaCl，主要水解产物为木二糖（X2）和木三糖（X3）。仅 4 小时后，复杂底物向短 XOS 的转化率约为 30%，葡糖醛木聚糖和黑麦阿拉伯木聚糖为 8.8%；而对于 PCC，Pp Xyn10A 大大提高了无支链 XOS 的产量。用来自山毛榉木葡糖醛木聚糖的 XOS 发酵B.teenis主要产生乙酸和乳酸。结构分析表明，虽然Pp Xyn10A 活性位点的糖基区域保存完好，但糖苷配基区域在 +2 亚位点具有芳香族相互作用，这可以解释为什么Pp Xyn10A 不释放木糖。