Layer-by-layer three-dimensional nanofibrous scaffolds (3DENS) were produced using the electrospinning technique. Interest in using biopolymers and application of electrospinning fabrication techniques to construct nanofibers for biomedical application has led to the development of scaffolds composed of PVA, keratin, and chitosan. To date, PVA/keratin blended nanofibers and PVA/chitosan blended nanofibers have been fabricated and studied for biomedical applications. Electrospun scaffolds comprised of keratin and chitosan have not yet been reported in published literature, thus a novel nanofibrous PVA/keratin/chitosan scaffold was fabricated by electrospinning. The resulting 3DENS were characterized using fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), differential scanning colorimetry (DSC), and thermogravimetric analysis (TGA). Physiochemical properties of the polymer solutions such as viscosity (rheology) and conductivity were also investigated. The 3DENS possess a relatively uniform fibrous structure, suitable porosity, swelling properties, and degradation which are affected by the mass ratio of keratin, and chitosan to PVA. These results demonstrate that PVA/keratin/chitosan 3DENS have the potential for biomedical applications.

使用静电纺丝技术生产逐层三维纳米纤维支架（3DENS）。对使用生物聚合物和应用静电纺丝制造技术来构建用于生物医学应用的纳米纤维的兴趣导致了由 PVA、角蛋白和壳聚糖组成的支架的开发。迄今为止，PVA/角蛋白混合纳米纤维和PVA/壳聚糖混合纳米纤维已经被制造和研究用于生物医学应用。由角蛋白和壳聚糖组成的静电纺丝支架尚未在已发表的文献中报道，因此通过静电纺丝制造了一种新型纳米纤维 PVA/角蛋白/壳聚糖支架。使用傅立叶变换红外 (FT-IR) 光谱、扫描电子显微镜 (SEM)、差示扫描比色法 (DSC)、和热重分析（TGA）。还研究了聚合物溶液的物理化学性质，例如粘度（流变学）和电导率。3DENS 具有相对均匀的纤维结构、合适的孔隙率、溶胀性和降解性，这些都受角蛋白和壳聚糖与 PVA 的质量比的影响。这些结果表明 PVA/角蛋白/壳聚糖 3DENS 具有生物医学应用的潜力。