Microbial colonization of catheter surfaces is responsible for most healthcare-associated infections. Quaternized chitin and chitosan have excellent antimicrobial and biocompatible properties and can be used to provide safe and prolonged protection for biomedical catheters. Herein, we prepared quaternized β-chitin derivative (QC)- and quaternized chitosan derivative (QCS)-based antimicrobial surfaces. The quaternized polysaccharides modified TPU surfaces exhibited hydrophilicity, good biocompatibility. Among these, QCS2-modified TPU exhibited excellent antibacterial properties against Gram-positive and Gram-negative bacteria, and prevented the adherence of bacteria compared with pristine TPU. The antibacterial activity of QCS2-modified surfaces maintained for 8 weeks under the condition of immersion in serum. An in vivo subcutaneous implantation experiment revealed 99.87% reduction of bacteria and reduced expression of inflammation-related factors in the surrounding tissue five days after implantation with QCS2-modified TPU. Therefore, quaternized polysaccharide-modified surfaces have promising potential in preventing medical catheter-associated infections.

导管表面的微生物定植是大多数医疗保健相关感染的原因。季铵化甲壳素和壳聚糖具有优异的抗菌和生物相容性，可用于为生物医学导管提供安全和长期的保护。在此，我们制备了基于季铵化 β-几丁质衍生物 (QC) 和季铵化壳聚糖衍生物 (QCS) 的抗菌表面。季铵化多糖改性的TPU表面表现出亲水性、良好的生物相容性。其中，与原始 TPU 相比，QCS2 修饰的 TPU 对革兰氏阳性和革兰氏阴性细菌表现出优异的抗菌性能，并防止细菌粘附。在浸入血清的条件下，QCS2修饰表面的抗菌活性可维持8周。体内皮下植入实验显示，在植入 QCS2 修饰的 TPU 5 天后，周围组织中的细菌减少了 99.87%，炎症相关因子的表达降低。因此，季铵化多糖改性表面在预防医用导管相关感染方面具有广阔的潜力。