Abstract

GH and DGAT1 are candidate genes associated with growth traits in sheep breeds. This study aims to determine the association between growth traits and GH and DGAT1 gene polymorphism using three statistical methods in Awassi sheep. The polymorphism was detected by the PCR-RFLP method. Three genotypes (AA, AB and BB) were observed for GH gene locus with allele and genotype frequency 0.70(A) and 0.30(B); 0.60(AA), 0.20(AB) and 0.20(BB). Three genotypes (CC, CT and TT) were found for DGAT1 gene locus with allele and genotype frequency 0.58(C) and 0.42(T); 0.47(CC), 0.23(CT) and 0.30(TT). The genes were in agreement with Hardy–Weinberg equilibrium (p > 0.05). CHAID, CRT and GLM were used to identify the association between growth traits and gene polymorphism. The results showed an association between GH locus and body weight and tail length. Also, found an association between DGAT1 locus and tail length. The three methods showed similar results in determining the association between genes and growth traits. Thus, the CHAID and CRT methods can evaluate the association between genes and growth traits.

摘要

GH 和 DGAT1 是与绵羊品种生长性状相关的候选基因。本研究旨在使用三种统计方法确定 Awassi 羊生长性状与 GH 和 DGAT1 基因多态性之间的关联。通过PCR-RFLP方法检测多态性。GH基因位点观察到三种基因型（AA、AB和BB），等位基因和基因型频率分别为0.70（A）和0.30（B）；0.60(AA)、0.20(AB) 和 0.20(BB)。DGAT1基因位点存在三种基因型（CC、CT和TT），等位基因和基因型频率分别为0.58（C）和0.42（T）；0.47（CC）、0.23（CT）和0.30（TT）。基因符合哈代-温伯格平衡（p > 0.05)。CHAID、CRT和GLM用于鉴定生长性状与基因多态性之间的关联。结果显示GH基因座与体重和尾长之间存在关联。此外，发现 DGAT1 基因座与尾长之间存在关联。这三种方法在确定基因与生长性状之间的关联方面显示出相似的结果。因此，CHAID 和 CRT 方法可以评估基因与生长性状之间的关联。