Abstract. The roles of microorganisms in enhancing crop production have been demonstrated for a range of cropping systems. Most studies to date, however, have been confined to a limited number of locations, making it difficult to identify general soil biotic and abiotic characteristics underpinning the yield-promotion across various locations. This knowledge gap limits our capacity to harness soil microbiome to improve crop production. Here we used high-throughput amplicon sequencing to investigate the common features of bacterial community composition, ecological networks and physicochemical properties in six yield-invigorating and adjacent yield-debilitating orchards. We found that yield-invigorating soils exhibited higher contents of organic matter than yield-debilitating soils and harboured unique bacterial communities. Greater alpha diversity and higher relative abundances of Planctomycetes and Chloroflexi were observed in yield-debilitating soils. Co-occurrence network analysis revealed that yield-invigorating soils displayed a greater number of meta-modules and a higher proportion of negative links to positive links. Chloroflexi was recognized as a keystone taxon in manipulating the interaction of bacterial communities in yield-invigorating soils. Structural equation modelling showed that soil organic matter, beta diversity of bacterial community, and network connector (Chloroflexi) were key factors supporting high-yield pear production. Altogether, we provide evidence that yield-invigorating soils across a range of locations appear to share common features, including accumulation of soil organic matter, higher microbial diversity, enrichment of key taxa like Chloroflexi, and maintaining a competitive network. These findings have implications for science-based guidance for sustainable food production.

中文翻译：

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有机质输入引发的土壤细菌群落支持梨的高产生产

摘要。微生物在提高作物产量方面的作用已在一系列种植系统中得到证实。然而，迄今为止的大多数研究都仅限于数量有限的地点，因此很难确定支持不同地点产量促进的一般土壤生物和非生物特征。这种知识差距限制了我们利用土壤微生物群来提高作物产量的能力。在这里，我们使用高通量扩增子测序来研究六个增产果园和相邻减产果园中细菌群落组成、生态网络和理化特性的共同特征。我们发现，增产土壤比减产土壤表现出更高的有机质含量，并拥有独特的细菌群落。在影响产量的土壤中观察到了更大的 alpha 多样性和更高的浮游菌和 Chloroflexi 的相对丰度。共生网络分析表明，增产土壤显示出更多的元模块和更高比例的负链接到正链接。Chloroflexi 被认为是控制增产土壤中细菌群落相互作用的关键分类群。结构方程模型表明，土壤有机质、细菌群落的 β 多样性和网络连接器 (Chloroflexi) 是支持高产梨生产的关键因素。总而言之，我们提供的证据表明，一系列地点的增产土壤似乎具有共同的特征，包括土壤有机质的积累、更高的微生物多样性、丰富像 Chloroflexi 这样的关键分类群，并保持一个有竞争力的网络。这些发现对可持续粮食生产的科学指导具有重要意义。