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Draft genome sequencing and functional annotation and characterization of biofilm-producing bacterium Bacillus novalis PD1 isolated from rhizospheric soil
Antonie van Leeuwenhoek ( IF 1.8 ) Pub Date : 2021-09-18 , DOI: 10.1007/s10482-021-01655-y
S Ishvaria 1 , Rajathirajan Siva Dharshini 1 , Ranjani Manickam 2 , K R Pooja 1 , Mohandass Ramya 1
Affiliation  

Biofilm forming bacterium Bacillus novalis PD1 was isolated from the rhizospheric soil of a paddy field. B. novalis PD1 is a Gram-positive, facultatively anaerobic, motile, slightly curved, round-ended, and spore-forming bacteria. The isolate B. novalis PD1 shares 98.45% similarity with B. novalis KB27B. B. vireti LMG21834 and B. drentensis NBRC 102,427 are the closest phylogenetic neighbours for B. novalis PD1. The draft genome RAST annotation showed a linear chromosome with 4,569,088 bp, encoding 6139 coding sequences, 70 transfer RNA (tRNA), and 11 ribosomal RNA (rRNA) genes. The genomic annotation of biofilm forming B. novalis PD1(> 3.6@OD595nm) showed the presence of exopolysaccharide-forming genes (ALG, PSL, and PEL) as well as other biofilm-related genes (comER, Spo0A, codY, sinR, TasA, sipW, degS, and degU). Antibiotic inactivation gene clusters (ANT (6)-I, APH (3′)-I, CatA15/A16 family), efflux pumps conferring antibiotic resistance genes (BceA, BceB, MdtABC-OMF, MdtABC-TolC, and MexCD-OprJ), and secondary metabolites linked to phenazine, terpene, and beta lactone gene clusters are part of the genome.



中文翻译:

从根际土壤中分离的产生物膜细菌新芽孢杆菌 PD1 的基因组测序草案和功能注释和表征

从稻田的根际土壤中分离出生物膜形成细菌新芽孢杆菌PD1。B. novalis PD1 是一种革兰氏阳性、兼性厌氧、能动、略弯曲、末端圆且能形成孢子的细菌。分离株新芽孢杆菌 PD1 与新芽孢杆菌KB27B具有 98.45% 的相似性 。B. vireti LMG21834 和B. drentensis NBRC 102,427 是B. novalis PD1最接近的系统发育邻居。基因组 RAST 注释草图显示了一条 4,569,088 bp 的线性染色体,编码 6139 个编码序列、70 个转移 RNA (tRNA) 和 11 个核糖体 RNA (rRNA) 基因。生物膜形成B. novalis的基因组注释PD1(> 3.6@OD 595nm ) 显示存在胞外多糖形成基因(ALG、PSL 和 PEL)以及其他生物膜相关基因(comER、Spo0A、codY、sinR、TasA、sipW、degS 和 degU) . 抗生素失活基因簇(ANT (6)-I、APH (3')-I、CatA15/A16 家族)、赋予抗生素抗性基因的外排泵(BceA、BceB、MdtABC-OMF、MdtABC-TolC 和 MexCD-OprJ)和与吩嗪、萜烯和 β 内酯基因簇相关的次生代谢物是基因组的一部分。

更新日期:2021-11-10
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