Grateloupia Livida polysaccharides-functionalized selenium nanoparticles (GLP-SeNPs) have been successfully prepared in a simple redox system of sodium selenite and ascorbic acid. The size, morphology, structure, stability and thermal behavior were analyzed by various characterization methods. These results showed that, GLP-SeNPs (particle size of 115.54 nm) prepared in optimal synthesis conditions (temperature of 45 °C, reaction time of 3 h, GLP concentration of 1.0 mg/mL and ascorbic acid concentration of 0.04 M) obtained by orthogonal experiments were uniform spherical and could be stable for 30 days at 4 °C. GLP-SeNPs exhibited significant scavenging ability on DPPH, ABTS, hydroxyl radical and superoxide anion radical when compared to GLP and Na₂SeO₃. GLP-SeNPs showed selective cytotoxicity toward various human cancer cells, but not normal cells. Besides, GLP-SeNPs exhibited low oral acute toxicity. Taken together, GLP-SeNPs might be used as potential diet nutritional supplement or anticancer agent.

Grateloupia Livida多糖功能化硒纳米粒子 (GLP-SeNPs) 已在亚硒酸钠和抗坏血酸的简单氧化还原系统中成功制备。通过各种表征方法分析了尺寸、形态、结构、稳定性和热行为。这些结果表明，在最佳合成条件（温度为 45 ℃，反应时间为 3 h，GLP 浓度为 1.0 mg/mL，抗坏血酸浓度为 0.04 M）下制备的 GLP-SeNPs（粒径为 115.54 nm）通过正交实验呈均匀球形，可在 4°C 下稳定 30 天。与 GLP 和 Na ₂ SeO ₃相比，GLP-SeNPs 对 DPPH、ABTS、羟基自由基和超氧阴离子自由基表现出显着的清除能力. GLP-SeNPs 对各种人类癌细胞表现出选择性的细胞毒性，但对正常细胞没有。此外，GLP-SeNPs 表现出较低的口服急性毒性。总之，GLP-SeNPs 可能被用作潜在的饮食营养补充剂或抗癌剂。