Transient receptor potential channel 6 (TRPC6) is a receptor-operated Ca²⁺ channel that plays an important role in Ca²⁺ influx in the majority of non–excitable cells and influences calcium signalling and cellular responses. Therefore, the purpose of the present study was to gain insight into the role of TRPC6 in the osteogenesis of periodontal ligament cells (PDLCs). By western blot and immunohistochemical staining, the protein level of TRPC6 was found to be increased in a time-dependent manner during osteoblastic differentiation of PDLCs. In addition, the TRPC6 inhibitor SKF96365 was used to block the function of TRPC6 and inhibit osteoblastic differentiation of PDLCs. The TRPC6 activator hyperforin dicyclohexylammonium salt (hyperforin DCHA) was used to activate TRPC6 and promote osteoblastic differentiation of PDLCs. In vivo, wild-type mice showed better bone regeneration than TRPC6^−/− mice, suggesting that TRPC6 has notable osteogenic induction properties and is important for bone defect repair. In conclusion, the current data demonstrated that TRPC6 plays a significant role in osteoblastic differentiation of PDLCs, suggesting that it may be a promising therapeutic target in osteogenesis.

瞬时受体电位通道 6 (TRPC6) 是一种受体操作的 Ca ^{2+通道，在 Ca 2+}中起重要作用流入大多数不可兴奋细胞并影响钙信号传导和细胞反应。因此，本研究的目的是深入了解 TRPC6 在牙周膜细胞 (PDLCs) 成骨中的作用。通过蛋白质印迹和免疫组织化学染色，发现在 PDLC 的成骨细胞分化过程中，TRPC6 的蛋白水平呈时间依赖性增加。此外，TRPC6抑制剂SKF96365用于阻断TRPC6的功能，抑制PDLCs的成骨细胞分化。TRPC6 激活剂 hyperforin 二环己基铵盐 (hyperforin DCHA) 用于激活 TRPC6 并促进 PDLC 的成骨细胞分化。在体内，野生型小鼠比 TRPC6 表现出更好的骨再生^-/-小鼠，表明 TRPC6 具有显着的成骨诱导特性，对骨缺损修复很重要。总之，目前的数据表明，TRPC6 在 PDLCs 的成骨细胞分化中起重要作用，表明它可能是成骨中一个有前途的治疗靶点。