Copper adhesive tape attached to the reverse side of a non-conductive glass slide to achieve protein MALDI-imaging in FFPE-tissue sections,Chemical Communications

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Copper adhesive tape attached to the reverse side of a non-conductive glass slide to achieve protein MALDI-imaging in FFPE-tissue sections
Chemical Communications ( IF 4.3 ) Pub Date : 2021-09-08 , DOI: 10.1039/d1cc03629g
Ran Wu _{1,

2} , Liang Qin ₂ , Lulu Chen ₂ , Rui Ma _{1,

2} , Difan Chen _{1,

2} , Haiqiang Liu _{1,

2} , Hualei Xu _{1,

2} , Hua Guo _{1,

2} , Yijun Zhou ₂ , Xiaodong Wang _{1,

2}

Affiliation

Herein, copper adhesive tape attached to the reverse side of a glass slide was developed as a new method to achieve protein in situ detection and imaging in a formalin fixed paraffin-embedded (FFPE) tissue section on a non-conductive glass slide by MALDI-MSI. The use of this new method led to 223 protein ions being imaged from a rat brain FFPE-tissue section on a non-conductive glass slide by MALDI-MS, compared to only 145 and 163 protein ions detected on an ITO glass slide and an AnchorChip target plate, respectively. This new method has great potential to become standard practice for protein MALDI-imaging in FFPE-tissue sections on non-conductive glass slides.

中文翻译：

贴在非导电玻璃载玻片背面的铜胶带，用于在 FFPE 组织切片中实现蛋白质 MALDI 成像

在此，贴在载玻片背面的铜胶带被开发为一种新方法，可通过 MALDI-在非导电载玻片上的福尔马林固定石蜡包埋 (FFPE) 组织切片中实现蛋白质原位检测和成像。微星。使用这种新方法可以通过 MALDI-MS 从非导电玻璃载玻片上的大鼠脑 FFPE 组织切片成像 223 个蛋白质离子，而在 ITO 载玻片和 AnchorChip 上仅检测到 145 和 163 个蛋白质离子目标板，分别。这种新方法具有成为非导电玻璃载玻片上 FFPE 组织切片中蛋白质 MALDI 成像的标准实践的巨大潜力。

更新日期：2021-09-20

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