Nontyphoidal Salmonella (NTS) is one of the major causes of bacterial foodborne infection. It is mainly manifested by self-limiting gastroenteritis in healthy individuals but can also cause severe complications including blood stream infection and mortality. The emergence of multidrug-resistant strains of Salmonella is becoming a global public health concern. This study is aimed at estimating the prevalence of Salmonella, identifying serotypes involved, and investigating antimicrobial susceptibility of the isolates among diarrheic patients attending private hospitals in Addis Ababa. We collected a total of 298 stool samples from diarrheic patients attending five private hospitals in Addis Ababa and isolated Salmonella according to standard microbiological techniques; the isolates were serotyped using slide agglutination and microplate agglutination techniques. Antimicrobial susceptibility test of the isolates was carried out using Kirby-Bauer disc diffusion assay according to Clinical Laboratory Standards Institute guidelines. Fourteen stool samples (4.7%) were positive for Salmonella, and Salmonella Kiambu was the most dominant serovar (, 50%) followed by S. Saintpaul (, 28.6%) and S. Haifa (, 14.3%). Three (21.4%) of the isolates were resistant to sulfisoxazole and tetracycline each and 2 (14.3%) to ampicillin. Resistance to two antimicrobials was detected only in 2 (14.3%) of the isolates, and none of the isolates were resistant to more than two antimicrobials. In conclusion, the current study showed low prevalence of NTS in diarrheic patients attending private hospitals in Addis Ababa. Although multidrug resistance to several antimicrobials was not detected in the isolates, prudent use of antimicrobials is recommended to guaranty the long-term use of the available antimicrobials.

中文翻译：

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埃塞俄比亚亚的斯亚贝巴私立医院腹泻患者的非伤寒沙门氏菌及其抗菌药物敏感性

非伤寒沙门氏菌(NTS) 是细菌性食源性感染的主要原因之一。它主要表现为健康个体的自限性胃肠炎，但也可能导致严重的并发症，包括血流感染和死亡。多重耐药沙门氏菌菌株的出现正在成为全球公共卫生问题。本研究旨在估计沙门氏菌的流行率，确定所涉及的血清型，并调查在亚的斯亚贝巴私立医院就诊的腹泻患者中分离物的抗菌素敏感性。我们从在亚的斯亚贝巴五家私立医院就诊的腹泻患者共收集了 298 份粪便样本并分离出沙门氏菌根据标准微生物技术；使用玻片凝集和微孔板凝集技术对分离物进行血清分型。根据临床实验室标准协会指南，使用 Kirby-Bauer 圆盘扩散试验对分离物进行抗菌药敏试验。14 份粪便样本 (4.7%)沙门氏菌呈阳性，而沙门氏菌Kiambu 是最主要的血清型。, 50%) 其次是S。圣保罗 （, 28.6%) 和S. Haifa (, 14.3%)。三个（21.4%）分离株分别对磺胺异恶唑和四环素耐药，2 个（14.3%）对氨苄青霉素耐药。仅在 2 个（14.3%）分离株中检测到对两种抗菌剂的耐药性，并且没有分离株对两种以上的抗菌剂产生耐药性。总之，目前的研究表明，在亚的斯亚贝巴私立医院就诊的腹泻患者中 NTS 的患病率较低。虽然在分离物中未检测到对几种抗菌药物的多重耐药性，但建议谨慎使用抗菌药物，以保证现有抗菌药物的长期使用。