Objective. To study the effect of pulsed radio frequency (PRF) on nerve repair and the expression of GFAP and GDNF in rats with neuropathic pain. Methods. Thirty SPF healthy SD rats were randomly divided into control group (Group C), PSNL group (partial ligation of sciatic nerve) + sham group (Group PS), and PSNL group (partial ligation of sciatic nerve) + PRF group (Group PR), with 10 rats in each group. In group C, the right sciatic nerve was exposed without ligation. In the PS group, the model of neuropathic pain was established by partial ligation of sciatic nerve. The mice in the PR group were treated with PRF after establishing the neuropathic pain model. The general behavior of rats during the treatment was observed. The mechanical and thermal hyperalgesia were measured before operation and 1, 3, 7, and 14 days after operation. The content of inflammatory factors in nerve tissue was detected by ELISA. The pathological condition of nerve tissue was observed by HE. The gene and protein changes of GFAP and GDNF in nerve tissue were determined by QRT PCR and Western blot. Results. Rats in the control group were free to move and in good condition. In the PS group, there were different degrees of claudication, weakness of the lower limbs, lateral toe valgus, nerve injury, and other behavioral changes. After the pulsed radiofrequency in the PR group, the above symptoms decreased gradually with the prolongation of the treatment time. The mechanical pain sensitivity and thermal allodynia of the PS group were reduced after the operation. The mechanical pain sensitivity and thermal pain sensitivity of the PR group gradually increased with the prolongation of the treatment time, and the 14 days were basically close to the control group. The levels of TNF-α and IL-6 in ELISA were significantly higher in the PS group than in the control group, and the content in the PR group was gradually reduced, which was close to the control group. HE staining showed that the sciatic nerve fibers disappeared, and the formation of nerve cavities was obvious in the 14-day PS group. The nerve fibers were found in the sciatic tissue of the PR group, and there was no obvious hemorrhagic edema and cell deformation. The expression of GFAP mRNA in the PS group was significantly higher than that in the control group and the PR group (), and the expression of GDNF was opposite (). The results of western blot showed that the expression of GFAP protein in the 14-day PS group was significantly higher than that in the control group. The expression of the PR group decreased compared with the control group, and the expression of GDNF was opposite (). Conclusion. Pulsed radiofrequency ablation can promote neurological repair, promote GDNF, and reduce the expression of GFAP in rats with neuropathic pain.

中文翻译：

---

脉冲射频对神经病理性疼痛大鼠神经修复及 GFAP 和 GDNF 表达的影响


客观的。研究脉冲射频（PRF）对神经病理性疼痛大鼠神经修复及GFAP、GDNF表达的影响。方法。 30只SPF级健康SD大鼠随机分为对照组（C组）、PSNL组（坐骨神经部分结扎）+假手术组（PS组）、PSNL组（坐骨神经部分结扎）+PRF组（PR组） ，每组 10 只大鼠。 C组暴露右侧坐骨神经，不结扎。 PS组采用部分结扎坐骨神经建立神经病理性疼痛模型。 PR组小鼠建立神经病理性疼痛模型后给予PRF治疗。观察治疗期间大鼠的一般行为。术前及术后1、3、7、14天测量机械和热痛觉过敏。采用ELISA法检测神经组织中炎症因子的含量。 HE观察神经组织病理情况。采用QRT PCR和Western blot检测神经组织中GFAP和GDNF基因和蛋白的变化。结果。对照组大鼠活动自如，状态良好。 PS组出现不同程度的跛行、下肢无力、脚趾外侧外翻、神经损伤等行为改变。 PR组脉冲射频治疗后，随着治疗时间的延长，上述症状逐渐减轻。 PS组术后机械痛敏感性和热异常性疼痛均降低。 PR组的机械痛敏感性和热痛敏感性随着治疗时间的延长逐渐升高，14天时基本接近对照组。 ELISA 中 TNF- α 、IL-6 含量 PS 组显着高于对照组，PR 组含量逐渐降低，与对照组接近。 HE染色显示PS 14 d组坐骨神经纤维消失，神经腔形成明显。 PR组坐骨组织内未见神经纤维，未见明显出血性水肿和细胞变形。 PS组GFAP mRNA表达量显着高于对照组和PR组（ ），而GDNF的表达则相反（ ）。 Western blot结果显示，PS 14 d组GFAP蛋白表达量显着高于对照组。 PR组较对照组表达减少，GDNF表达相反（ ）。结论。脉冲射频消融可以促进神经病理性疼痛大鼠的神经修复、促进GDNF、减少GFAP的表达。