4D microstructural changes in dentinal tubules during acid demineralisation,Dental Materials

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4D microstructural changes in dentinal tubules during acid demineralisation
Dental Materials ( IF 4.6 ) Pub Date : 2021-09-20 , DOI: 10.1016/j.dental.2021.09.002
Nathanael Leung ₁ , Robert A Harper ₂ , Bin Zhu ₁ , Richard M Shelton ₂ , Gabriel Landini ₂ , Tan Sui ₁

Affiliation

Objective

Dental erosion is a common oral condition caused by chronic exposure to acids from intrinsic/extrinsic sources. Repeated acid exposure can lead to the irreversible loss of dental hard tissues (enamel, dentine, cementum). Dentine can become exposed to acid following severe enamel erosion, crown fracture, or gingival recession. Causing hypersensitivity, poor aesthetics, and potential pulp involvement. Improving treatments that can restore the structural integrity and aesthetics are therefore highly desirable. Such developments require a good understanding of how acid demineralisation progresses where relatively little is known in terms of intertubular dentine (ITD) and peritubular dentine (PTD) microstructure. To obtain further insight, this study proposes a new in vitro method for performing demineralisation studies of dentine.

Methods

Advanced high-speed synchrotron X-ray microtomography (SXM), with high spatial (0.325 μm) and temporal (15 min) resolution, was used to conduct the first in vitro, time-resolved 3D (4D) study of the microstructural changes in the ITD and PTD phases of human dentine samples (∼0.8 × 0.8 × 5 mm) during 6 h of continuous acid exposure.

Results

Different demineralisation rates of ITD (1.79 μm/min) and PTD (1.94 μm/min) and their progressive width-depth profiles were quantified, which provide insight for understanding the mechanisms of dentine demineralisation.

Significance

Insights obtained from morphological characterisations and the demineralisation process of ITD and PTD during acid demineralisation would help understand the demineralisation process and potentially aid in developing new therapeutic dentine treatments. This method enables continuous examination of relatively large volumes of dentine during demineralisation and also demonstrates the potential for studying the remineralisation process of proposed therapeutic dentine treatments.

中文翻译：

酸脱矿过程中牙本质小管的 4D 显微结构变化

客观的

牙齿侵蚀是一种常见的口腔疾病，由长期暴露于内在/外在来源的酸引起。反复接触酸会导致牙齿硬组织（牙釉质、牙本质、牙骨质）的不可逆损失。在严重的牙釉质侵蚀、牙冠断裂或牙龈萎缩后，牙本质会暴露在酸中。导致过敏、不美观和潜在的牙髓受累。因此，非常需要改进可以恢复结构完整性和美观的处理方法。这种发展需要很好地了解酸脱矿是如何进行的，而在管间牙本质 (ITD) 和管周牙本质 (PTD) 微观结构方面知之甚少。为了获得更深入的了解，本研究提出了一种新的体外方法来进行牙本质脱矿研究。