Abstract

Artemisia vulgaris essential oil (AVEO) is known as a therapeutic agent. Several types of investigation have demonstrated its cytotoxic impact on human cancer cells. In the current study, AVEO nanoemulsion (AVEO-NE) was synthesized using an ultrasound-based emulsification method and its cytotoxic, apoptotic, antioxidant, and anti-angiogenic properties were evaluated for the MCF-7 cancer cell line by measuring the cell’s gene expression profile and performing MTT. Finally, the anti-angiogenic activity of AVEO-NE was verified by applying CAM assay analysis. The results showed that AVEO-NE exhibited significant cell-selective cytotoxic, apoptotic, and antioxidant activities by up-regulating Cas-9, CAT, and SOD gene expression. Moreover, the AVEO-NE meaningfully suppressed angiogenesis in MCF-7 cancer cells by down-regulating VEGF gene expression. Finally, the decreased number and length of chick embryo chorioallantoic membrane (CAM) blood vessels verified AVEO-NE’s anti-angiogenic activity. Therefore, AVEO-NE can potentially be employed as a safe cell-dependent anti-breast cancer drug delivery system.

摘要

青蒿精油（AVEO）被称为治疗剂。几种类型的研究已经证明了它对人类癌细胞的细胞毒性影响。在目前的研究中，AVEO 纳米乳 (AVEO-NE) 是使用基于超声的乳化方法合成的，并通过测量细胞的基因表达来评估其对 MCF-7 癌细胞系的细胞毒性、凋亡、抗氧化和抗血管生成特性。配置文件并执行 MTT。最后，通过应用CAM测定分析验证了AVEO-NE的抗血管生成活性。结果表明，AVEO-NE 通过上调 Cas-9、CAT 和 SOD 基因的表达，表现出显着的细胞选择性细胞毒、凋亡和抗氧化活性。此外，AVEO-NE 通过下调 VEGF 基因表达有效地抑制了 MCF-7 癌细胞中的血管生成。最后，鸡胚绒毛尿囊膜 (CAM) 血管数量和长度的减少证实了 AVEO-NE 的抗血管生成活性。因此，AVEO-NE 有可能被用作一种安全的细胞依赖性抗乳腺癌药物递送系统。