The psocid Liposcelis entomophila has developed strong pesticide resistance and is outbroken frequently, thus seriously threatening food security. Understanding the function of vitellogenin (Vg) and the hormone regulation in L. entomophila will help in obtaining comprehensive knowledge about its reproductive processes and developing new pest management strategies via reproduction disruption. The full-length cDNA of Vg in L. entomophila (LeVg) contains a 5799-bp open reading frame encoding a 1932 amino acid protein. LeVg belongs to the large lipid transfer protein (LLTP) superfamily and has conserved domains and feature motifs of insect Vgs. LeVg is primarily expressed in the fat body of female adults. Notably, feeding on in vitro synthesized dsLeVg significantly suppressed LeVg transcription level. This resulted in inadequately developed ovarioles and reduced fecundity and egg hatchability, suggesting a critical role for vitellogenin in L. entomophila reproduction. Moreover, transcription levels of LeVg and its receptor LeVgR increased in virgin female adults after topical treatment with of 23 ng JH III/insect. This study provides preliminary results about the vitellogenesis of Psocoptera insects and demonstrates that Vg could serve as a promising target for RNAi-based population management of psocids.

psocid Liposcelis entomophila具有很强的抗药性，爆发频繁，严重威胁粮食安全。了解卵黄蛋白原 (Vg) 的功能和L. entomophila 中的激素调节将有助于获得有关其繁殖过程的全面知识，并通过繁殖中断制定新的害虫管理策略。全长cDNA的Vg的在L. entomophila（LeVg）含有编码1932氨基酸蛋白的5799-bp的开放阅读框。LeVg 属于大型脂质转移蛋白 (LLTP) 超家族，具有昆虫 Vgs 的保守域和特征基序。左值主要在成年女性的脂肪体中表达。值得注意的是，以体外合成的 ds LeVg 为食可显着抑制LeVg转录水平。这导致子房发育不充分，繁殖力和卵孵化率降低，表明卵黄蛋白原在L. entomophila繁殖中起关键作用。此外，在用 23 ng JH III/昆虫局部治疗后，LeVg及其受体LeVgR 的转录水平在处女雌性成虫中增加。本研究提供了关于 Psooptera 昆虫卵黄发生的初步结果，并证明了Vg 可以作为基于 RNAi 的 psocids 种群管理的有希望的目标。