Fibrous scaffold along with seed cells are essential players for engineered tissue regeneration. Recently, PLGA/epoxidized poly(isoprene) dense membranes have been evaluated for cell growth and have shown satisfactory results. However, porous and fibrous structures suitable for obtaining 3D supports have not yet been evaluated for the PLGA/epoxidized poly(isoprene). The present work aimed to establish the electrospinning conditions for obtaining electrospun membranes with a smaller diameter of fibers and adequate morphology, which were characterized in vitro by their physical, chemical and biological properties. The best electrospun fibers were obtained from the following conditions: an applied voltage of 15 kV, a needle-collector distance of 20 cm and, a flow rate of 5 mL/h. The functional groups of the polymers involved in the blend did not show any changes. The mechanical properties of the electrospun membranes are within the lower limits known to human skin and some soft tissues. The in vitro degradation test showed a loss of mass of approximately 20% in 28 days. Significant adhesion and proliferation of human adipose–derived mesenchymal stem cells were demonstrated, indicating that there was cellular penetration into the scaffold and proliferation. Therefore, the preliminary results suggest that the electrospun PLGA/epoxidized poly(isoprene) membranes have high potential for application as a 3D tissue engineering scaffold.

纤维支架和种子细胞是工程组织再生的重要参与者。最近，已对 PLGA/环氧化聚（异戊二烯）致密膜进行了细胞生长评估，并显示出令人满意的结果。然而，尚未对 PLGA/环氧化聚（异戊二烯）适用于获得 3D 支撑的多孔和纤维结构进行评估。目前的工作旨在建立静电纺丝条件，以获得具有较小直径纤维和适当形态的静电纺丝膜，其在体外通过其物理、化学和生物特性进行表征。从以下条件获得最好的电纺纤维：施加的电压为 15 kV，针收集器距离为 20 cm，流速为 5 mL/h。共混物中涉及的聚合物的官能团没有显示任何变化。静电纺丝膜的机械性能在人类皮肤和一些软组织已知的下限内。体外降解试验表明，28 天内质量损失约 20%。证明了人脂肪来源的间充质干细胞的显着粘附和增殖，表明细胞渗透到支架中并增殖。因此，初步结果表明，电纺 PLGA/环氧化聚（异戊二烯）膜作为 3D 组织工程支架具有很高的应用潜力。体外降解试验表明，28 天内质量损失约 20%。证明了人脂肪来源的间充质干细胞的显着粘附和增殖，表明细胞渗透到支架中并增殖。因此，初步结果表明，电纺 PLGA/环氧化聚（异戊二烯）膜作为 3D 组织工程支架具有很高的应用潜力。体外降解试验表明，28 天内质量损失约 20%。证明了人脂肪来源的间充质干细胞的显着粘附和增殖，表明细胞渗透到支架中并增殖。因此，初步结果表明，电纺 PLGA/环氧化聚（异戊二烯）膜作为 3D 组织工程支架具有很高的应用潜力。