Abstract

In the present study, the extracted volatiles from dried leaf and fruit of Piper longum were analysed by gas chromatography-mass spectrometry (GC-MS) and each detected 53 constituents having 92.41% and 96.31% of the total volatiles respectively. E-nerolidol (19.56%), β-pinene (17.07%) and α-pinene (6.8%) were main constituents in leaf volatiles whereas the fruit volatiles dominated by germacrene-D (23.38%), 8-heptadecene (8.95%) and β-caryophyllene (8.20%). Antioxidant potential of the volatiles were assessed by DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) assays. The fruit volatiles revealed higher radical scavenging activities as compared to leaf. The samples were also evaluated against multidrug resistant (MDR) isolates including one non MDR fungal strain. The fruit volatiles showed a very strong activity against Acinetobacter baumannii than others whereas leaf volatiles possessed strong activity against Klebsiella pneumoniae as compared to other strains. Thus, the dried fruits can be exploited for drug development towards therapeutic purpose.

摘要

本研究采用气相色谱-质谱（GC-MS）分析了荜茇干叶和果实中提取的挥发物，各检测出53种成分，分别占总挥发物的92.41%和96.31%。E-橙花油（19.56%）、β-蒎烯（17.07%）和α-蒎烯（6.8%）是叶片挥发物中的主要成分，而果实挥发物则以germacrene-D（23.38%）、8-十七碳烯（8.95%）为主和β-石竹烯 (8.20%)。通过 DPPH (1,1-diphenyl-2-picrylhydrazyl) 和 ABTS (2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) 测定评估挥发物的抗氧化潜力。与叶子相比，果实挥发物显示出更高的自由基清除活性。还针对包括一种非 MDR 真菌菌株的多重耐药 (MDR) 分离株对样品进行了评估。果实挥发物对鲍曼不动杆菌的活性比其他菌株强，而叶挥发物对肺炎克雷伯菌的活性比其他菌株强。因此，干果可用于药物开发以达到治疗目的。