Boronic Acid Substituted Polyaniline Based Enzymatic Biosensor System for Catechol Detection,Electroanalysis

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Boronic Acid Substituted Polyaniline Based Enzymatic Biosensor System for Catechol Detection
Electroanalysis ( IF 2.7 ) Pub Date : 2021-09-20 , DOI: 10.1002/elan.202100271
Zeycan Kalkan ₁ , Merve Yence ₂ , Fatih Turk ₁ , Tamer Bektas ₁ , Soner Ozturk ₂ , Sedat Surdem ₂ , Nimet Yildirim Tirgil ₂

Affiliation

In this research, an electrochemical enzymatic biosensor system for sensitive and selective catechol detection was developed with using boric acid substituted Polyaniline film coated two different electrodes; gold screen-printed electrode and glassy carbon electrode. Electropolymerization of 3-aminophenylboronic acid is carried out through various electropolymerization conditions, and the best conditions for biosensors systems are concluded. The developed tyrosinase enzyme-based biosensor system is used for the determination of catechol. The limit of detections(LOD) of the developed biosensor was 0.25 μM and 1.8 μM with Au SPE and GCE systems, respectively. Additionally, real sample analysis was also performed for controlled catechol added green tea samples.

中文翻译：

用于邻苯二酚检测的硼酸取代聚苯胺酶生物传感器系统

在这项研究中，开发了一种用于灵敏和选择性检测儿茶酚的电化学酶生物传感器系统，使用硼酸取代的聚苯胺薄膜涂覆两个不同的电极；金丝网印刷电极和玻碳电极。3-氨基苯基硼酸的电聚合通过各种电聚合条件进行，总结出生物传感器系统的最佳条件。开发的基于酪氨酸酶的生物传感器系统用于测定儿茶酚。所开发的生物传感器的检测限 (LOD) 分别为 Au SPE 和 GCE 系统的 0.25 μM 和 1.8 μM。此外，还对添加了受控儿茶酚的绿茶样品进行了实际样品分析。

更新日期：2021-09-20

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