The medicinal plants industry, particularly in regard to products rich in biologically active substances for maintaining health, has grown by leaps and bounds in the last decade, with sales of over-the-counter drugs containing these substances growing by billions of dollars. Attention has thus also been paid to the safety and effectiveness of these medicines. We are currently witnessing a rapid increase in the number of publications devoted to the development of new separation procedures that are not only fast and cheap but also more efficient and eco-friendlier, improving both yields and quality of extracts quality without using hazardous organic solvents. The new approaches include those that use deep eutectic solvents (DES), which are characterized by unique parameters. In fact, DESs can be used for both the isolation and determination of biologically active substances in medicinal plants. Therefore, the purpose of the review is to gather details on the application of DESs in the separation of bioactive compounds in medicinal plants and to provide a solid background for future research in this area. To cover these aspects, the available data and references in the field of interest are reviewed and summarized.

药用植物行业，特别是富含用于维持健康的生物活性物质的产品，在过去十年中突飞猛进，含有这些物质的非处方药的销售额增长了数十亿美元。因此，这些药物的安全性和有效性也受到了关注。目前，我们见证了致力于开发新分离程序的出版物数量迅速增加，这些程序不仅快速、廉价，而且更高效、更环保，在不使用危险有机溶剂的情况下提高了提取物的产量和质量。新方法包括使用具有独特参数的深共熔溶剂 (DES) 的方法。实际上，DES 可用于药用植物中生物活性物质的分离和测定。因此，本综述的目的是收集有关 DES 在药用植物中生物活性化合物分离中应用的详细信息，并为该领域的未来研究提供坚实的背景。为了涵盖这些方面，审查和总结了感兴趣领域中的可用数据和参考资料。