Aromatase and nonaromatase neurons in the zebra finch secondary auditory forebrain are indistinct in their song-driven gene induction and intrinsic electrophysiological properties,European Journal of Neuroscience

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Aromatase and nonaromatase neurons in the zebra finch secondary auditory forebrain are indistinct in their song-driven gene induction and intrinsic electrophysiological properties
European Journal of Neuroscience ( IF 2.7 ) Pub Date : 2021-09-17 , DOI: 10.1111/ejn.15463
Catherine Bournonville ₁ , Kyssia Ruth Mendoza ₁ , Luke Remage‐Healey ₁

Affiliation

Estrogens support major brain functions including cognition, reproduction, neuroprotection and sensory processing. Neuroestrogens are synthesized within some brain areas by the enzyme aromatase and can rapidly modulate local circuit functions, yet the cellular physiology and sensory-response profiles of aromatase neurons are essentially unknown. In songbirds, social and acoustic stimuli drive neuroestrogen elevations in the auditory forebrain caudomedial nidopallium (NCM). In both males and females, neuroestrogens rapidly enhance NCM auditory processing and auditory learning. Estrogen-producing neurons in NCM may therefore exhibit distinguishing profiles for sensory-activation and intrinsic electrophysiology. Here, we explored these questions using both immunocyctochemistry and electrophysiological recordings. Immunoreactivity for aromatase and the immediate early gene EGR1, a marker of activity and plasticity, were quantified in NCM of song-exposed animals versus silence-exposed controls. Using whole-cell patch clamp recordings from NCM slices, we also documented the intrinsic excitability profiles of aromatase-positive and aromatase-negative neurons. We observed that a subset of aromatase neurons were significantly activated during song playback, in both males and females, and in both hemispheres. A comparable population of non-aromatase-expressing neurons were also similarly driven by song stimulation. Membrane properties (i.e., resting membrane potential, rheobase, input resistance and multiple action potential parameters) were similarly indistinguishable between NCM aromatase and non-aromatase neurons. Together, these findings demonstrate that aromatase and non-aromatase neurons in NCM are indistinct in terms of their intrinsic electrophysiology and responses to song. Nevertheless, such similarities in response properties may belie more subtle differences in underlying conductances and/or computational roles that may be crucial to their function.

中文翻译：

斑胸草雀次级听觉前脑中的芳香化酶和非芳香化酶神经元在其歌曲驱动的基因诱导和内在电生理学特性方面并不清楚

雌激素支持主要的大脑功能，包括认知、生殖、神经保护和感觉处理。神经雌激素由芳香酶在某些大脑区域合成，可以快速调节局部回路功能，但芳香酶神经元的细胞生理学和感觉反应谱基本上是未知的。在鸣禽中，社交和声学刺激会驱动听觉前脑尾内侧核皮质 (NCM) 中的神经雌激素升高。在男性和女性中，神经雌激素迅速增强 NCM 听觉处理和听觉学习。因此，NCM 中产生雌激素的神经元可能表现出不同的感觉激活和内在电生理学特征。在这里，我们使用免疫细胞化学和电生理记录探索了这些问题。在暴露于歌曲的动物与暴露于沉默的对照的 NCM 中，对芳香化酶和立即早期基因 EGR1（一种活性和可塑性的标志物）的免疫反应性进行了量化。使用来自 NCM 切片的全细胞膜片钳记录，我们还记录了芳香化酶阳性和芳香化酶阴性神经元的内在兴奋性特征。我们观察到在歌曲播放期间，男性和女性以及两个半球的一部分芳香酶神经元都被显着激活。类似的非芳香酶表达神经元群也同样受到歌曲刺激的驱动。NCM 芳香酶和非芳香酶神经元之间的膜特性（即静息膜电位、rheobase、输入电阻和多个动作电位参数）同样难以区分。一起，这些发现表明 NCM 中的芳香酶和非芳香酶神经元在其内在电生理学和对歌曲的反应方面是模糊的。然而，响应特性的这种相似性可能掩盖了潜在电导和/或计算角色的更细微差异，这可能对其功能至关重要。

更新日期：2021-11-10

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