Herein, a facile protocol of simple DNA adsorption on UV-initiated polymerization supports was proposed for effectively fabricating aptamer-based affinity monolithic column. Hydrophilic cationic monolith with an excellent mechanical stability was achieved within 7 min and then massive aptamers were directly bound by DNA charge-dependent adsorption. Strong cationic quaternary ammonium-based monomer was employed to provide effective and stable positive charge surface for aptamer immobilization in a wide range of pH. An ultra-high aptamer coverage density of 6813 pmol/μL was achieved to gain a highly specific online recognition performance. Limitations such as low aptamer capacity, tedious modification and time-consuming reactions in the traditional biological or covalent modification strategies were avoided. By using ochratoxin A (OTA) as the given analyte, the selective recognition and high recoveries were successfully achieved, and little cross-reactivity towards OTB analogue was only 0.5% even if the content of OTB got up to 125 folds of OTA. Applied to sample analysis, the satisfactory discriminations of trace OTA were obtained at 93.9 ± 1.9% – 96.5 ± 1.7%（n = 3）in beer, wheat and chicken liver samples. It might light a cost-effective access to efficiently preparing high-performance affinity monoliths towards the selective in-tube microextraction of OTA.

在此，提出了一种简单的 DNA 吸附在 UV 引发的聚合载体上的简便方案，用于有效制造基于适体的亲和整体柱。在 7 分钟内实现了具有优异机械稳定性的亲水性阳离子整料，然后大量适体通过 DNA 电荷依赖性吸附直接结合。采用强阳离子季铵基单体为适配体在广泛的 pH 范围内提供有效且稳定的正电荷表面。实现了 6813 pmol/μL 的超高适配体覆盖密度，以获得高度特异性的在线识别性能。避免了传统生物或共价修饰策略中适体容量低、修饰繁琐和反应耗时等限制。通过使用赭曲霉毒素 A (OTA) 作为给定的分析物，成功实现了选择性识别和高回收率，即使 OTB 含量高达 OTA 的 125 倍，对 OTB 类似物的交叉反应性也很小，仅为 0.5%。应用于样品分析，在啤酒、小麦和鸡肝样品中，痕量 OTA 的鉴别率为 93.9 ± 1.9% – 96.5 ± 1.7%（n = 3）。它可能会为高效制备高性能亲和单体提供一种具有成本效益的途径，以实现 OTA 的选择性管内微萃取。9 ± 1.9% – 96.5 ± 1.7%（n = 3）在啤酒、小麦和鸡肝样品中。它可能会为高效制备高性能亲和单体提供一种具有成本效益的途径，以实现 OTA 的选择性管内微萃取。9 ± 1.9% – 96.5 ± 1.7%（n = 3）在啤酒、小麦和鸡肝样品中。它可能会为高效制备高性能亲和性单体提供一种经济高效的途径，以实现 OTA 的选择性管内微萃取。