A study of the effect of bioagents and dipotassium phosphate (DPP) and their combination on early blight disease reduction under greenhouse conditions was conducted. Native bacterial isolates as bio-control agents exhibited control against early blight. Five bacterial isolates were tested against the pathogen. All isolates exhibited significant antagonistic activity against Alternariasolani, isolate “bact-03” showed significant in vitro inhibition (42.6%) and later was identified as Bacillus amyloliquefaciensby 16S rDNA gene analysis. Tests conducted on dipotassium phosphate (DPP) at different concentrations (10 mM, 25 mM, and 50 mM) showed mycelial growth inhibition 14.2%, 27.4%, and 54.8%, respectively. In vitro synergetic study on seed germination showed that the combination of DPP and B. amyloliquefaciens antagonized the pathogen. Vigor index was also significant in the combination (343.0) as well as DPP (299.0), and bioagent (426.6) compared to control (170.0). In the in vivo application of B. amyloliquefaciens, DPP combination showed significant disease reduction. However, disease severity on the plants treated with DPP was 35%, and the plants treated with B. amyloliquefaciens was 30% while in combination of both showed the disease severity up to 42% that was significantly lower than control (82%). Application of these bioagents and DPP also sustained the plant weight by promoting the growth and development of plant. The results of this study indicate that naturally existing bioagents along with the slats of potassium phosphate may provide promising control of early blight disease.. Due to their antagonistism, bacterial strains with the combination of salts can be used as bio-pesticides. Their applicationsimproves seed health and crop yield, but signaling relationship in pathogens, plants, and soil still needs to divulge to promote BCAs as encouraging bio-pesticides for the future.

对生物制剂和磷酸二钾 (DPP) 及其组合在温室条件下对减少早疫病病害的影响进行了研究。作为生物防治剂的天然细菌分离物表现出对早疫病的控制。针对病原体测试了五种细菌分离物。所有分离株均表现出对链格孢菌的显着拮抗活性，分离株“bact-03”显示出显着的体外抑制作用（42.6%），后来被鉴定为解淀粉芽孢杆菌通过 16S rDNA 基因分析。对不同浓度（10 mM、25 mM 和 50 mM）的磷酸二钾 (DPP) 进行的测试显示，菌丝体生长抑制分别为 14.2%、27.4% 和 54.8%。种子萌发的体外协同研究表明，DPP和解淀粉芽孢杆菌的组合可以拮抗病原体。与对照 (170.0) 相比，组合 (343.0) 以及 DPP (299.0) 和生物制剂 (426.6) 的活力指数也很显着。在解淀粉芽孢杆菌的体内应用中，DPP 组合显示出显着的疾病减少。然而，用 DPP 处理的植物的疾病严重程度为 35%，用解淀粉芽孢杆菌处理的植物为 30%，而两者结合显示疾病严重程度高达 42%，显着低于对照 (82%)。这些生物制剂和 DPP 的应用还通过促进植物的生长和发育来维持植物重量。这项研究的结果表明，天然存在的生物制剂与磷酸钾盐一起可以为早疫病提供有希望的控制。由于它们的拮抗作用，具有盐组合的细菌菌株可用作生物农药。它们的应用改善了种子健康和作物产量，但仍需要透露病原体、植物和土壤中的信号关系，以促进 BCA 作为未来的生物农药。