In this study, we aimed to investigate the taxonomy and various characteristics of Dunaliella salina IBSS-2 strain and describe its cultivation potential in mid-latitude climate during springtime. In addition, our analysis confirmed the essentiality of combining morphological, physiological, and other characteristics when identifying new species and strains of the genus Dunaliella, along with the molecular marker (internal transcribed spacer (ITS) of rDNA gene). The pilot cultivation of microalgae during the springtime in the south of Russia demonstrated that the climatic conditions of this region allow D. salina cultivation for biomass accumulation during this season, highlighting light and temperature conditions as the main factors determining the growth rate of D. salina. A two-fold increase in daily insolation and, consequently, in temperature in April resulted in a more than three-fold increase in productivity of D. salina culture. The maximum productivity of D. salina both in April and May was comparable and reached 2 g m⁻² day⁻¹, and the total yield for 8–10 days was about 14.5–16 g m⁻². The additional CO₂ supply into the D. salina culture did not show any significant effect on its growth rate; however, it contributed to maintaining the diversity of morphometric characteristics over a longer period of time. Changes in the morphological and morphometric characteristics of algal cells, including size reduction, were observed during the batch cultivation. Thus, the production potential of the green carotenogenic microalga D. salina was determined in the springtime, which allows expanding the seasonal interval of its cultivation in temperate latitudes.

在本研究中，我们旨在调查杜氏盐藻IBSS-2 菌株的分类学和各种特性，并描述其在春季中纬度气候条件下的栽培潜力。此外，我们的分析证实了在鉴定杜氏藻属新物种和菌株时结合形态学、生理学和其他特征以及分子标记（rDNA 基因的内部转录间隔区 (ITS)）的重要性。在俄罗斯南部春季进行的微藻试点培养表明，该地区的气候条件允许盐生藻在这个季节进行生物量积累的栽培，强调光和温度条件是决定盐藻生长速度的主要因素。每日日照增加两倍，因此四月份的温度增加了三倍多，导致盐藻养殖的生产力增加了三倍多。D. salina 4、5月份的最大产量相当，达到2 g m ^-2  day ^-1，8-10天的总产量约为14.5-16 g m ^-2。额外的 CO ₂供应到D. salina文化对其生长速度没有任何显着影响；然而，它有助于在较长时间内保持形态特征的多样性。在分批培养过程中观察到藻类细胞的形态和形态特征的变化，包括尺寸减小。因此，绿色类胡萝卜微藻D. salina的生产潜力是在春季确定的，这可以扩大其在温带地区栽培的季节间隔。