Dickeya species are causal agents of soft rot diseases in many economically important crops, including soft rot disease of potato (Solanum tuberosum). Using random barcode transposon-site sequencing (RB-TnSeq), we generated genome-wide mutant fitness profiles of Dickeya dadantii 3937, Dickeya dianthicola ME23, and Dickeya dianthicola 67-19 isolates collected after passage through several in vitro and in vivo conditions. Tubers from the potato cultivars “Atlantic”, “Dark Red Norland”, and “Upstate Abundance” provided highly similar conditions for bacterial growth. Using the homolog detection software PyParanoid, we matched fitness values for orthologous genes in the three bacterial strains. Direct comparison of fitness among the strains highlighted shared and variable traits important for growth. Bacterial growth in minimal medium required many metabolic traits that were also essential for competitive growth in planta, such as amino acid, carbohydrate, and nucleotide biosynthesis. Growth in tubers specifically required the pectin degradation gene kduD. Disruption in three putative DNA-binding proteins had strain-specific effects on competitive fitness in tubers. Though the Soft Rot Pectobacteriaceae can cause disease with little host specificity, it remains to be seen the extent to which strain-level variation impacts virulence.

中文翻译：

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全基因组鉴定对马铃薯（Solanum tuberosum）块茎中 Dickeya dadantii 和 D. dianthicola 的生长很重要的基因

Dickeya物种是许多重要经济作物中软腐病的病原体，包括马铃薯 ( Solanum tuberosum )软腐病。使用随机条形码座子现场测序（RB-TnSeq），我们产生的全基因组突变体健身型材的Dickeya dadantii 3937，的Dickeya dianthicola ME23，和的Dickeya dianthicola通过几个传代后收集67-19株在体外和体内状况。来自马铃薯品种“大西洋”、“深红色诺兰”和“北部丰度”的块茎为细菌生长提供了高度相似的条件。使用同源检测软件 PyParanoid，我们匹配了三种细菌菌株中直系同源基因的适合度值。菌株之间适应性的直接比较突出了对生长重要的共享和可变特征。细菌在基本培养基中的生长需要许多代谢特性，这些特性对于植物的竞争性生长也是必不可少的，例如氨基酸、碳水化合物和核苷酸的生物合成。块茎的生长特别需要果胶降解基因kduD。三种假定的 DNA 结合蛋白的破坏对块茎的竞争适应性具有菌株特异性影响。虽然软腐Pectobacteriaceae可引起宿主特异性很小的疾病，菌株水平变异对毒力的影响程度仍有待观察。