We used an LC-MS/MS metabolomics approach to investigate one-carbon metabolism in the plasma of flaxseed-fed White Leghorn laying hens (aged 3.5 years). In our study, dietary flaxseed (via the activity of a vitamin B6 antagonist known as “1-amino d-proline”) induced at least 15-fold elevated plasma cystathionine. Surprisingly, plasma homocysteine (Hcy) was stable in flaxseed-fed hens despite such highly elevated cystathionine. To explain stable Hcy, our data suggest accelerated Hcy remethylation via BHMT and MS-B12. Also supporting accelerated Hcy remethylation, we observed elevated S-adenosylmethionine (SAM), an elevated SAM:SAH ratio, and elevated methylthioadenosine (MTA), in flaxseed-fed hens. These results suggest that flaxseed increases SAM biosynthesis and possibly increases polyamine biosynthesis. The following endpoint phenotypes were observed in hens consuming flaxseed: decreased physiological aging, increased empirical lifespan, 9–14% reduced body mass, and improved liver function. Overall, we suggest that flaxseed can protect women from ovarian tumor metastasis by decreasing omental adiposity. We also propose that flaxseed protects cancer patients from cancer-associated cachexia by enhancing liver function.

中文翻译：

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亚麻籽通过有毒地增强一碳代谢来延长动物寿命并降低卵巢癌的严重程度


我们使用 LC-MS/MS 代谢组学方法研究了亚麻籽喂养的白来航蛋鸡（3.5 岁）血浆中的一碳代谢。在我们的研究中，膳食亚麻籽（通过称为“1-氨基d-脯氨酸”的维生素 B6 拮抗剂的活性）可导致血浆胱硫醚升高至少 15 倍。令人惊讶的是，尽管胱硫醚含量如此之高，但在亚麻籽喂养的母鸡中，血浆同型半胱氨酸 (Hcy) 却很稳定。为了解释稳定的 Hcy，我们的数据表明通过 BHMT 和 MS-B12 加速 Hcy 再甲基化。我们还观察到，在亚麻籽喂养的母鸡中，S-腺苷甲硫氨酸 (SAM) 升高，SAM:SAH 比率升高，甲硫腺苷 (MTA) 升高，这也支持 Hcy 再甲基化加速。这些结果表明，亚麻籽增加了 SAM 生物合成，并可能增加了多胺生物合成。在食用亚麻籽的母鸡中观察到以下终点表型：生理衰老减少，经验寿命延长，体重减少 9-14%，肝功能改善。总的来说，我们认为亚麻籽可以通过减少网膜肥胖来保护女性免受卵巢肿瘤转移。我们还提出，亚麻籽可以通过增强肝功能来保护癌症患者免受癌症相关恶病质的侵害。