DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.

中文翻译：

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DPY19L3 参与 C2C12 成肌细胞的成肌分化

DPY19L3 已被鉴定为用于血小板反应蛋白 1 型重复结构域的蛋白质的C-甘露糖基转移酶。在这项研究中，我们专注于 DPY19L3 在 C2C12 小鼠成肌细胞成肌分化中的作用。我们使用 CRISPR/Cas9 系统进行了 DPY19L3 基因去除。结果显示这些DPY19L3敲除细胞不能被诱导分化。此外，与亲本细胞相比，DPY19L3 敲除细胞中 MEK/ERK 和 p70S6K 的磷酸化水平受到抑制，这表明作为 DPY19L3 介导的C-甘露糖基化并调节 MEK的蛋白质/ERK 或 p70S6K 信号是分化所必需的。